Effects of dietary vitamin K3 supplementation on vitamin K1 and K2 (menaquinone) dynamics in dairy cows
Effects of dietary vitamin K3 supplementation on vitamin K1 and K2 (menaquinone) dynamics in dairy cows
复制标题
日粮中补充维生素 K3 对奶牛维生素 K1 和 K2（甲基萘醌）动态的影响
DOI：
10.1111/asj.13680
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发表时间：
2022
影响因子：
2
通讯作者：
Obara Yoshiaki
中科院分区：
文献类型：
--
作者：
Bai Hanako;Arai Hikoji;Ikuta Kentarou;Ishikawa Sho;Ohtani Yoshihisa;Iwashita Kunihiro;Okada Nao;Shirakawa Hitoshi;Komai Michio;Terada Fuminori;Obara Yoshiaki
膳食维生素K3（VK 3）对反刍动物的影响尚未得到充分研究。本研究的目的是研究日粮VK 3对奶牛泌乳性能、瘤胃特性以及VK 1和甲基萘醌（MK或VK 2）在瘤胃、血浆和牛奶中动态的影响。在两个交叉试验中使用了8头泌乳后期的荷斯坦奶牛，包括对照（未处理）和50或200 mg/天（d）VK 3补充组。14天后，采集血浆、瘤胃液和乳汁，并使用荧光高效液相色谱法（HPLC）测定其VK 1和MK含量。饲喂50 mg/天VK 3后，产奶量保持不变，但饲喂200 mg/天VK 3后，产奶量略有下降。饲喂200 mg/天VK 3后，瘤胃液中丙酸盐的摩尔比显著增加。此外，VK 3喂养（50和200 mg/天）后，血浆和乳汁中的MK-4浓度均显著增加。在瘤胃液中，饲喂200 mg/天VK 3后MK-4浓度增加。这些结果表明，VK 3可能是荷斯坦奶牛泌乳后期MK-4（VK的生物活性形式）的良好来源。本研究为了解VK在奶牛中的生理作用提供了依据。
The effect of dietary vitamin K3(VK3) on ruminant animals is not fully investigated. The aim of this study was to examine the effects of dietary VK3 on lactation performance, rumen characteristics, and VK1 and menaquinone (MK, or VK2) dynamics in the rumen, plasma, and milk of dairy cows. Eight Holstein dairy cows in late lactation periods were used in two crossover trials including a control (nontreatment) and a 50 or 200 mg/day (d) VK3 supplementation group. After 14 days, plasma, ruminal fluid, and milk were sampled and their VK1 and MKs contents were measured using fluorescence‐high‐performance liquid chromatography (HPLC). Milk production was unchanged after feeding 50 mg/day VK3 but marginally decreased after feeding 200 mg/day VK3. The molar ratio of propionate in ruminal fluid was significantly increased on feeding 200 mg/day VK3. Additionally, MK‐4 concentrations significantly increased in both plasma and milk after VK3 feeding (50 and 200 mg/day). In ruminal fluid, MK‐4 concentrations increased after 200 mg/day VK3 feeding. These results suggest that VK3 may be a good source of MK‐4, the biologically active form of VK, in Holstein dairy cows during their late lactation periods. This study provides a basis for understanding the physiological role of VK in dairy cows.