Direct Laser Writing for Deterministic Lateral Displacement of Submicron Particles
Direct Laser Writing for Deterministic Lateral Displacement of Submicron Particles
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DOI：
10.1109/jmems.2020.2998958
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发表时间：
2020-10-01
影响因子：
2.7
通讯作者：
Sochol, Ryan D.
中科院分区：
文献类型：
--
作者：
Alsharhan, Abdullah T.;Stair, Anthony J.;Sochol, Ryan D.
新兴的增材制造（或“三维（3D）打印”）策略提供了极大扩展现有微流体技术能力的潜力。例如，“确定性横向位移（DLD）”的操作性能--一种其中微/纳米柱排列在微流体通道内使得能够将目标悬浮颗粒被动运输远离其初始流动流的技术--是基于几何设计变量，诸如排列的柱之间的差距间距（G）。对于涉及亚微米级颗粒的DLD处理的应用（例如，细胞外囊泡），然而，通过常规的微制造方案实现必要的几何控制代表了技术上具有挑战性的制造障碍。为了绕过这些障碍，在这里，我们探索使用双光子“直接激光写入（DLW）”的增材制造DLD阵列能够亚微米颗粒处理。DLW制造条件的研究表明，增加激光功率从22.5 mW到27.5 mW显着降低G从1.51 +/- 0.04 μ m到1.02 +/- 0.05 μ m，分别。在DLW打印的DLD系统内的直径为860 nm的荧光颗粒的实验性微流体测试揭示了颗粒沿着成角度排列的微柱的有效流体动力学栏杆，在500 μ m的通道长度上具有15.3 +/-8.6 μ m的横向位移。据我们所知，这些结果代表了能够处理亚微米颗粒的3D打印DLD系统的第一份报告，从而为基于DLW的DLD生物医学应用提供了有希望的基础。[2020-0123]
Emerging additive manufacturing (or "three-dimensional (3D) printing") strategies offer the potential to vastly extend the capabilities of established microfluidic technologies. For example, the operational performance of "deterministic lateral displacement (DLD)" - a technique in which micro/nanoposts arrayed inside of a microfluidic channel enable passive transport of target suspended particles away from their initial flow streams - is based on geometric design variables, such as the gap spacing between the arrayed posts (G). For applications that involve DLD processing of submicron-scale particles (e.g., extracellular vesicles), however, achieving the requisite geometric control via conventional microfabrication protocols represents a technically challenging manufacturing hurdle. To bypass such barriers, here we explore the use of two-photon "direct laser writing (DLW)" for additively manufacturing DLD arrays capable of submicron particle handling. Studies of DLW fabrication conditions revealed that increasing the laser power from 22.5 mW to 27.5 mW significantly decreased G from 1.51 +/- 0.04 mu m to 1.02 +/- 0.05 mu m, respectively. Experimental microfluidic testing of 860 nm-indiameter fluorescent particles within the DLW-printed DLD system revealed effective hydrodynamic railing of particles along the angled arrayed microposts, with a lateral displacement of 15.3 +/- 8.6 mu m over a channel length of 500 mu m. These results represent, to our knowledge, the first report of a 3D printed DLD system capable of processing submicron particles, thereby offering a promising foundation for DLW-enabled DLD-based biomedical applications. [2020-0123]