Identification of botanicals and potential contaminants through RFLP and sequencing.
Identification of botanicals and potential contaminants through RFLP and sequencing.
复制标题
DOI:
10.1055/s-2005-871230
复制
发表时间:
2005-07
期刊:
影响因子:
2.7
通讯作者:
M. Lum;Ellie Potter;Thanh Dang;D. Heber;M. Hardy;A. Hirsch
中科院分区:
文献类型:
--
作者:
M. Lum;Ellie Potter;Thanh Dang;D. Heber;M. Hardy;A. Hirsch
Botanical supplements for health enhancement are being increasingly used in the United States, but no safeguards are formally in place to ensure that they are not contaminated with non-efficacious or potentially harmful plant material. A molecular approach, which allows the authentication of botanical ingredients and detection of contaminating plant material by analyzing the ITS-1 region by PCR-RFLP and subsequent sequencing, is described. When using starting material from which DNA can be obtained, this method has the potential for identifying both primary and contaminating plant material in botanical dietary supplements.