COL10A1 expression is elevated in diverse solid tumor types and is associated with tumor vasculature
COL10A1 expression is elevated in diverse solid tumor types and is associated with tumor vasculature
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DOI：
10.2217/fon.12.79
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发表时间：
2012-08-01
期刊：
影响因子：
3.3
通讯作者：
West, Michael D.
中科院分区：
文献类型：
--
作者：
Chapman, Karen B.;Prendes, Maria J.;West, Michael D.
目的：鉴定在多种肿瘤类型中上调的分子标志物可能导致新的诊断和治疗策略。作者筛选了一组从不同肿瘤和肿瘤细胞系中制备的RNA，并将其与正常组织和培养的体细胞类型进行比较，以鉴定在广谱肿瘤类型中表达的候选基因。材料和方法：对代表20多种肿瘤类型的128个个体肿瘤样品、代表31种不同正常组织类型的85个样品、68个肿瘤细胞系和97种不同正常原代细胞培养物进行基因表达微阵列分析。相对于正常组织，在大量和多种肿瘤中对基因的表达升高进行排名。结果与结论：COL 10A 1被鉴定为在大多数正常组织中具有限制性表达并且在许多不同肿瘤类型中表达升高的基因。相比之下，在本研究中调查的68个肿瘤细胞系中未检测到COL 10A 1表达。免疫荧光研究定位胶原，X型，α-1（胶原X）染色的肿瘤血管在乳腺肿瘤，而正常乳腺组织的血管是胶原X-阴性或有显着较低的水平。X型胶原蛋白的肿瘤微环境特异性表达及其在血管系统中的定位可能有助于其作为诊断和治疗不同实体瘤类型的新靶点。
Aim: The identification of molecular markers that are upregulated in multiple tumor types could lead to novel diagnostic and therapeutic strategies. The authors screened a panel of RNAs prepared from diverse tumors and tumor cell lines, and compared them with normal tissues and cultured somatic cell types, in order to identify candidate genes expressed in a broad spectrum of tumor types. Materials & methods: Gene expression microarray analysis was carried out on 128 individual tumor samples representing over 20 tumor types, 85 samples representing 31 diverse normal tissue types, 68 tumor cell lines and 97 diverse normal primary cell cultures. Genes were ranked for elevated expression across a large number and variety of tumors relative to normal tissues. Results & conclusion: COL10A1 was identified as a gene with restricted expression in most normal tissues and elevated expression in many diverse tumor types. By contrast, COL10A1 expression was undetectable in the 68 tumor cell lines surveyed in this study. Immunofluorescence studies localized collagen, type X, alpha-1 (collagen X) staining to tumor vasculature in breast tumors, whereas the vasculature of normal breast tissue was either collagen X-negative or had markedly lower levels. The tumor microenvironment-specific expression of collagen X, together with its localization in the vasculature, may facilitate its use as a novel target for the diagnosis and treatment of diverse solid tumor types.