Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells.
Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells.
复制标题
白色念珠菌生物膜、菌丝和酵母形成细胞的细胞表面削片。
DOI：
10.1002/pmic.201100588
复制
发表时间：
2012
期刊：
影响因子：
3.4
通讯作者：
Chaffin,WLaJean
中科院分区：
文献类型：
--
作者：
Vialas,Vital;Perumal,Palani;Gutierrez,Dolores;Ximenez-Embun,Pilar;Nombela,Cesar;Gil,Concha;Chaffin,WLaJean
我们使用简单的胰蛋白酶处理，然后使用纳米 LC 进行肽分离和鉴定，然后使用离线 MS/MS 来鉴定在生物膜和浮游酵母细胞和菌丝中生长的活白色念珠菌生物体的表面蛋白。一百三十一个蛋白质存在于一种条件的三个重复中的至少两个中，并且分布在三种生长条件的各种组合中。先前报道的表面蛋白和新的表面蛋白都被鉴定出来，并且它们分布在细胞壁的共价连接蛋白和非共价连接蛋白之间。
We used a brief trypsin treatment followed by peptide separation and identification using nano‐LC followed by off‐line MS/MS to identify the surface proteins on liveCandida albicansorganisms growing in biofilms and planktonic yeast cells and hyphae. One hundred thirty‐one proteins were present in at least two of the three replicates of one condition and distributed in various combinations of the three growth conditions. Both previously reported and new surface proteins were identified and these were distributed between covalently attached proteins and noncovalently attached proteins of the cell wall.