RELATIONSHIPS BETWEEN THE PHYSICAL AND GENETIC MAPS OF A 470X10(3) BASE-PAIR REGION AROUND THE TERMINUS OF ESCHERICHIA-COLI-K12 DNA-REPLICATION
RELATIONSHIPS BETWEEN THE PHYSICAL AND GENETIC MAPS OF A 470X10(3) BASE-PAIR REGION AROUND THE TERMINUS OF ESCHERICHIA-COLI-K12 DNA-REPLICATION
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DOI：
10.1016/0022-2836(82)90414-4
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发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
LOUARN, JM
中科院分区：
文献类型：
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作者：
BOUCHE, JP;GELUGNE, JP;LOUARN, JM
在E. coliK 12 DNA复制（Bouche，1982）与遗传标记att.vphi.80、trpABCDE、fnr、rac、trg和man有关，trp和man之间有46 kb（酶）/min，表明接合转移在末端区域没有减慢。使用这种关系，trq定位到31.4分钟，发现rac从29.6延伸到30.1分钟。E.同源遗传元件coli K12（Kaiser，1980），将被称为kim，在图上在34.2至34.6分钟处被鉴定。测量在同步复制循环结束时标记的片段的比活性。它们表明，对于所研究的trp：：噬菌体Mu菌株，最终标记的DNA在31.2 ±。0.2在该位置两侧延伸50 kb的区域中，DNA复制可能被延迟或减慢。
The physical map of the region on both sides of the terminus of E. coli K12 DNA replication (Bouche, 1982) was related to the genetic markers att.vphi.80, trpABCDE, fnr, rac, trg and man. There are 46 kb (kilobases)/min between trp and man, indicating that conjugative transfer is not slowed down in the region of the terminus. Using this relationship, trq was mapped to 31.4 min and rac was found to extend from 29.6 to 30.1 min. The 3rd phage .lambda.-homologous genetic element of E. coli K12 (Kaiser, 1980), to be called kim, was identified on the map at 34.2 to 34.6 min. The specific activities of fragments labeled at the end of a synchronized replication cycle were measured. They indicate that, for the trp::phage Mu strain studied, the ultimately labeled DNA is at 31.2 .+-. 0.2 min. DNA replication may be delayed or slowed down in a region extending 50 kb on either side of this position.