RanGAP-mediated nucleocytoplasmic transport of Prospero regulates neural stem cell lifespan in Drosophila larval central brain
RanGAP-mediated nucleocytoplasmic transport of Prospero regulates neural stem cell lifespan in Drosophila larval central brain
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DOI：
10.1111/acel.12854
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发表时间：
2019-02-01
期刊：
影响因子：
7.8
通讯作者：
Yang, Xiaohang
中科院分区：
文献类型：
--
作者：
Wu, Di;Wu, Litao;Yang, Xiaohang
在果蝇蛹脑神经母细胞（NBs）的神经发生结束时，细胞核普洛斯普洛斯（Pros）触发细胞周期退出并终止脑神经母细胞的寿命。在这里，我们揭示了在幼虫脑NBs中，一种内在机制通过ran介导的核细胞质运输系统促进了Pros在核膜上的进出口。在rangap突变体中，Pros从细胞核向细胞质的输出受到损害，并且Pros的核质运输变成单向运输，导致Pros在幼虫中央脑NBs的细胞核中早期积累。这种核pro保留启动NB细胞周期退出，导致NB总数过早减少。我们的数据表明，RanGAP在神经发生过程中控制NB寿命的内在机制中起着至关重要的作用。我们的研究可能为理解其他生物神经发生过程中神经干细胞的寿命提供见解。
By the end of neurogenesis in Drosophila pupal brain neuroblasts (NBs), nuclear Prospero (Pros) triggers cell cycle exit and terminates NB lifespan. Here, we reveal that in larval brain NBs, an intrinsic mechanism facilitates import and export of Pros across the nuclear envelope via a Ran-mediated nucleocytoplasmic transport system. In rangap mutants, the export of Pros from the nucleus to cytoplasm is impaired and the nucleocytoplasmic transport of Pros becomes one-way traffic, causing an early accumulation of Pros in the nuclei of the larval central brain NBs. This nuclear Pros retention initiates NB cell cycle exit and leads to a premature decrease of total NB numbers. Our data indicate that RanGAP plays a crucial role in this intrinsic mechanism that controls NB lifespan during neurogenesis. Our study may provide insights into understanding the lifespan of neural stem cells during neurogenesis in other organisms.