Genetic variability and phylogenetic analysis of hosta virus X
Genetic variability and phylogenetic analysis of hosta virus X
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DOI：
10.1007/s00705-009-0522-6
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发表时间：
2009-10
影响因子：
2.7
通讯作者：
O. Fajolu;R.-H. Wen;A. Windham;M. Windham;J. Moulton;M. R. Hajimorad
中科院分区：
文献类型：
--
作者：
O. Fajolu;R.-H. Wen;A. Windham;M. Windham;J. Moulton;M. R. Hajimorad
测定了30个来自美国田纳西州的玉簪X病毒（HVX）分离物的TGB 1和CP基因序列，并与GenBank中的序列进行了比较。所有已知的HVX分离物（包括来自TN的那些）的CP分别具有98.3-100%和98.2-100%的核苷酸和氨基酸序列同一性，而TGB 1具有97.4-100%的核苷酸和97-100%的氨基酸序列同一性。TN分离物的TGB 1都比韩国分离物长一个密码子，这是唯一公开可用的序列。系统发育分析的核苷酸和氨基酸序列的TGB 1和CP的所有已知的HVX分离物，单独或组合，揭示了密切的关系，这表明它们都来自一个共同的祖先。系统发育分析证实HVX是马铃薯X病毒属的一个独立种。
Triple gene block 1 (TGB1) and coat protein (CP) sequences of 30 hosta virus X (HVX) isolates from Tennessee (TN), USA, were determined and compared with available sequences in GenBank. The CPs of all known HVX isolates, including those from TN, shared 98.3–100% and 98.2–100% nucleotide and amino acid sequence identity, respectively, whereas TGB1 shared 97.4–100% nucleotide and 97–100% amino acid sequence identity. TGB1 of TN isolates were all longer by one codon from that of a Korean isolate, which is the only sequence publicly available. Phylogenetic analysis of nucleotide and amino acid sequences of TGB1 and CP of all known HVX isolates, separately or combined, revealed a close relationship, suggesting that all of them are derived from a common ancestor. Phylogenetic analysis with the type member of each genus of the familyFlexiviridaeconfirmed that HVX is a member of a distinct species of the genusPotexvirus.