A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria.
A Multicenter Study of Beta-Lactamase Resistant Escherichia coli and Klebsiella pneumoniae Reveals High Level Chromosome Mediated Extended Spectrum β Lactamase Resistance in Ogun State, Nigeria.
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DOI：
10.1155/2014/819896
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发表时间：
2014
影响因子：
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通讯作者：
Agunlejika RA
中科院分区：
文献类型：
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作者：
Adeyankinnu FA;Motayo BO;Akinduti A;Akinbo J;Ogiogwa JI;Aboderin BW;Agunlejika RA
由于多重耐药细菌的问题日益严重，我们制定了一项监测计划，目的是确定环境中ESBL的基本分子特性。选取197株大肠埃希菌和肺炎克雷伯菌进行ESBL生产和药敏试验。质粒谱的测定和固化能力的测试。所有分离株的ESBL患病率为26.4%，大肠杆菌的比例更高。对氨苄西林、四环素和复方三甲松均有绝对耐药。对第二代和第三代头孢菌素的敏感性高于平均水平。分离株质粒谱范围为9 ~ 26 kbp，大肠杆菌平均为14.99±2.3 kbp，肺炎克雷伯菌平均为20.98±1.8 kbp， ESBL阳性大肠杆菌质粒治愈率为9.6%，肺炎克雷伯菌质粒治愈率为3.9%。目前的研究表明革兰氏阴性菌获得ESBL的数量激增，并且有证据表明不同亚型的ESBL与质粒可传播亚型和染色体编码亚型共循环。这就要求对这种公共卫生治疗进行普遍监测，并在分子流行病学方面作出更多努力。
As a result of the ever increasing problem of multiresistant bacteria, we instituted a surveillance program with the aim of identifying the basic molecular properties of ESBL in our environment. About 197 isolates of Escherichia coli and Klebsiella pneumoniae were selected and tested for ESBL production and antimicrobial susceptibility. Plasmid profiles were determined and curing ability was tested. ESBL prevalence was 26.4% for all isolates tested, with E. coli having a greater proportion. There was absolute resistance to ampicilin, tetracycline, and co-trimaxole among tested isolates. There was above average susceptibility to the 2nd and 3rd generation cephalosporins. Plasmid profiles of tested isolates ranged from 9 kbp to 26 kbp with average of 14.99 ± 2.3 kbp for E. coli and 20.98 ± 1.8 kbp K. pneumoniae, 9.6% of ESBL positive E. coli plasmids were cured, while 3.9% of K. pneumoniae plasmids were cured after treatment. The present study shows an upsurge in ESBL acquisition by gram negative bacteria and evidence of cocirculation of varying subtypes of ESBL with both plasmid transmissible and chromosome encoded subtypes. This calls for universal surveillance and more effort towards molecular epidemiology of this public health treatment.