Analysis of expression of drug resistance-linked ABC transporters in cancer cells by quantitative RT-PCR.
Analysis of expression of drug resistance-linked ABC transporters in cancer cells by quantitative RT-PCR.
复制标题
通过定量 RT-PCR 分析癌细胞中耐药相关 ABC 转运蛋白的表达。
DOI：
10.1007/978-1-60761-700-6_6
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Ambudkar,SureshV
中科院分区：
文献类型：
--
作者：
Calcagno,AnnaMaria;Ambudkar,SureshV
Quantitative real-time PCR (qRT-PCR) boasts many advantages over microarrays. For instance, very low amounts of total RNA are required to yield highly accurate and reproducible detection of transcript levels. As a consequence, qRT-PCR has become a popular technique for assessing gene expression levels and is now the gold standard. In this chapter, qRT-PCR using two distinct chemistries, SYBR Green and TaqMan, are described. We compare ABC transporter levels in various drug-resistant cancer cell lines by employing each method. SYBR Green yields reproducible results; nevertheless, TaqMan chemistry is superior to SYBR Green, as it displays higher specificity and sensitivity. Gene expression analysis by qRT-PCR is a powerful technique and shows potential as a diagnostic tool for predicting drug response in cancer patients.
影响因子：
50.3
作者：
Szakács, G;Annereau, JP;Gottesman, MM
通讯作者：
Gottesman, MM
影响因子：
2.7
作者：
Wittwer, CT;Herrmann, MG;Rasmussen, RP
通讯作者：
Rasmussen, RP
影响因子：
3.6
作者：
Annereau, JP;Szakacs, G;Gottesman, MM
通讯作者：
Gottesman, MM