Slc26a11, a chloride transporter, localizes with the vacuolar H(+)-ATPase of A-intercalated cells of the kidney.
Slc26a11, a chloride transporter, localizes with the vacuolar H(+)-ATPase of A-intercalated cells of the kidney.
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Slc26a11 是一种氯离子转运蛋白，与肾脏 A 嵌入细胞的液泡 H(+)-ATP 酶一起定位。
DOI：
10.1038/ki.2011.196
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发表时间：
2011
影响因子：
19.6
通讯作者：
Soleimani,Manoocher
中科院分区：
文献类型：
--
作者：
Xu,Jie;Barone,Sharon;Li,Hong;Holiday,Shannon;Zahedi,Kamyar;Soleimani,Manoocher
氯离子在调节跨特化细胞膜和细胞内膜的液泡H+-ATP酶活性方面具有重要作用。在肾脏中，空泡H+-ATP酶表达于集合管中泌酸A型闰细胞的顶膜上，在其中其在酸分泌和全身酸碱稳态中具有重要作用。在这里，我们报告的氯离子转运蛋白，共定位和调节质膜H+-ATP酶在肾脏集合管的活性的鉴定。免疫印迹和免疫荧光标记鉴定了Slc 26 a11（约72 kDa），在集合管中的细胞亚群中表达。基于与AQP 2的双重免疫荧光标记和与H+-ATP酶的相同共定位，表达Slc 26 a11的细胞被认为不同于主细胞，并且被发现是嵌入细胞。在瞬时转染的COS 7细胞中的功能研究表明，Slc 26 a11（命名为肾脑阴离子转运蛋白（KBAT））可以通过H+-ATP酶转运氯离子并增加酸排出速率。因此，Slc 26 a11是液泡H+-ATP酶的伴侣，促进集合管中的酸分泌。
Chloride has an important role in regulating vacuolar H+-ATPase activity across specialized cellular and intracellular membranes. In the kidney, vacuolar H+-ATPase is expressed on the apical membrane of acid-secreting A-type intercalated cells in the collecting duct where it has an essential role in acid secretion and systemic acid base homeostasis. Here, we report the identification of a chloride transporter, which co-localizes with and regulates the activity of plasma membrane H+-ATPase in the kidney collecting duct. Immunoblotting and immunofluorescent labeling identified Slc26a11 (∼72 kDa), expressed in a subset of cells in the collecting duct. On the basis of double-immunofluorescent labeling with AQP2 and identical co-localization with H+-ATPase, cells expressing Slc26a11 were deemed to be distinct from principal cells and were found to be intercalated cells. Functional studies in transiently transfected COS7 cells indicated that Slc26a11 (designated as kidney brain anion transporter (KBAT)) can transport chloride and increase the rate of acid extrusion by means of H+-ATPase. Thus, Slc26a11 is a partner of vacuolar H+-ATPase facilitating acid secretion in the collecting duct.