Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin
Comparative analysis of the D genome-encoded high-molecular weight subunits of glutenin
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DOI：
10.1007/s00122-005-0051-y
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发表时间：
2005-09
影响因子：
5.4
通讯作者：
Y. Wan;Zehong Yan;Kunfan Liu;Youliang Zheng;R. D'Ovidio;P. Shewry;Nigel G Halford;Daowen Wang
中科院分区：
文献类型：
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作者：
Y. Wan;Zehong Yan;Kunfan Liu;Youliang Zheng;R. D'Ovidio;P. Shewry;Nigel G Halford;Daowen Wang
利用聚合酶链反应（PCR）技术从节节麦和普通小麦中扩增出4个编码1Dx型高分子量（HMW）亚基的基因。这两个亚基来自Ae。tauschii（1Dx2.1t和1Dx2t）与普通小麦1Dx2亚基的序列非常相似。与此相反，两个新的面包小麦亚基（1Dx2.2和1Dx2.2*）不同于亚基1Dx2在其重复结构域内具有不同的内部重复区域（分别为132和186个氨基酸）。这些重复序列位于它们被重复的区域附近，并且在每个末端具有完整的完整重复基序。这些结果的HMW亚基进化和小麦品质改良的意义进行了讨论。
Four genes encoding novel 1Dx-type high-molecular weight (HMW) subunits were amplified by polymerase chain reaction, two each fromAegilops tauschiiand bread wheatTriticum aestivum. The two subunits fromAe. tauschii(1Dx2.1tand 1Dx2t) were both very similar in sequence to subunit 1Dx2 from bread wheat. In contrast, the two novel bread wheat subunits (1Dx2.2 and 1Dx2.2*) differed from subunit 1Dx2 in having different internally duplicated regions (of 132 and 186 amino acid, respectively) within their repetitive domains. These duplicated sequences were located adjacent to the regions from which they had been duplicated and had complete intact repeat motifs at each end. The implications of these results for HMW subunit evolution and wheat quality improvement are discussed.