All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody
All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody
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DOI：
10.1016/0092-8674(81)90383-4
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发表时间：
1981-12
期刊：
影响因子：
64.5
通讯作者：
R. Pruss;R. Mirsky;M. Raff;R. Thorpe;A. Dowding;B. Anderton
中科院分区：
文献类型：
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作者：
R. Pruss;R. Mirsky;M. Raff;R. Thorpe;A. Dowding;B. Anderton
我们已经产生了一种单克隆抗体，其在免疫荧光测定中与所有类别的中间丝反应，包括星形胶质细胞中的神经胶质丝、轴突中的神经丝、上皮PtK 2细胞中的张力丝和成纤维细胞中的中间丝。它还结合到骨骼肌中的2条线上。在SDS-聚丙烯酰胺凝胶中，抗体与大多数甚至可能所有先前定义的主要中间丝蛋白结合，包括神经胶质纤维酸性蛋白、三种脊椎动物神经丝蛋白（“神经丝三联体”）、波形蛋白、结蛋白、几种细胞角蛋白以及鱿鱼和海洋蠕虫粘液虫的神经丝蛋白。此外，抗体结合到一种分子量约为66的蛋白质，DDD可能是所有中间丝的组分。这些发现表明，所有脊椎动物和无脊椎动物的中间丝蛋白共享一个共同的抗原决定簇，并提高了所有中间丝包含66，DDD分子量蛋白的可能性。
We have produced a monoclonal antibody that reacts with all classes of intermediate filaments in immunofluorescence assays, including glial filaments in astrocytes, neurofilaments in axons, tonofilaments in epithelial PtK2 cells and intermediate filaments in fibroblasts. It also binds to 2 lines in skeletal muscle. In SDS-polyacrylamide gels, the antibody binds to most and perhaps all of the major intermediate filament proteins that have been previously defined, including glial fibrillary acidic protein, the three vertebrate neurofilament proteins (the “neurofilament triplet”), vimentin, desmin, several cytokeratins and the neurofilament proteins of squid and the marine worm Myxicola. In addition, the antibody binds to a protein with an approximate molecular weight of 66, DDD that may be a component of all intermediate filaments. These findings suggest that all vertebrate and invertebrate intermediate filament proteins share a common antigenie determinant and raise the possibility that all intermediate filaments contain a 66, DDD molecular weight protein.