Mycobacterium tuberculosis complex DNA in calcified pleura from remains 1400 years old
Mycobacterium tuberculosis complex DNA in calcified pleura from remains 1400 years old
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1400 年前遗骸钙化胸膜中的结核分枝杆菌复合体 DNA
DOI：
10.1046/j.1472-765x.1998.00436.x
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发表时间：
1998
影响因子：
2.4
通讯作者：
D. Minnikin
中科院分区：
文献类型：
--
作者：
H. Donoghue;M. Spigelman;J. Zias;A. M. Gernaey‐Child;D. Minnikin
应用聚合酶链反应技术，从1400年前的钙化胸膜中分离鉴定了结核分枝杆菌复合体DNA。这是第一次在非木乃伊化的考古组织而不是骨骼中证明结核病;样本中存在分枝杆菌分枝菌酸支持这一结论。对微生物病原体的古老DNA的研究是令人感兴趣的，因为它可以验证传统诊断，可以回答疾病史上长期存在的问题，并提供可以与现代分离物进行比较的古老DNA序列。
Mycobacterium tuberculosis complex DNA was isolated and identified in calcified pleura from remains 1400 years old, with the polymerase chain reaction. This is the first demonstration of tuberculosis in non‐mummified archaeological tissue other than bone; the presence of mycobacterial mycolic acids in the sample supports this conclusion. The study of ancient DNA from microbial pathogens is of interest as it enables verification of traditional diagnoses, may answer long‐standing questions in the history of disease, and provides ancient DNA sequences that can be compared with those of modern isolates.