Photophysical properties of acene DCDHF fluorophores: Long-wavelength single-molecule emitters designed for cellular Imaging
Photophysical properties of acene DCDHF fluorophores: Long-wavelength single-molecule emitters designed for cellular Imaging
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DOI：
10.1021/jp0712598
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发表时间：
2007-09-20
影响因子：
2.9
通讯作者：
Moerner, W. E.
中科院分区：
文献类型：
--
作者：
Lord, Samuel J.;Lu, Zhikuan;Moerner, W. E.
我们报告了荧光团 DCDHF-N-6 和 DCDHF-A-6 的溶剂化变色、粘度敏感和单分子光物理学。这些分子属于二氰基亚甲基二氢呋喃 (DCDHF) 类单分子发射体，包含通过共轭单元连接的胺电子供体和 DCDHF 受体； DCDHF-N-6 和 DCDHF-A-6 分别具有萘和蒽共轭连接体。这些分子保持了亚苯基连接的 DCDHF 的有益光物理学（即光稳定性、发射波长对溶剂极性的依赖性以及量子产率对溶剂粘度的敏感性），同时提供更适合细胞成像的较长波长的吸收和发射。我们证明，与其他几种常用染料（罗丹明 6G、德克萨斯红和荧光素）相比，这些新荧光团在水性环境中的光稳定性较低。最后，我们对在活细胞质膜中扩散的并苯 DCDHF 的单个副本进行成像。
We report the solvatochromic, viscosity-sensitive, and single-molecule photophysics of the fluorophores DCDHF-N-6 and DCDHF-A-6. These molecules are members of the dicyanomethylenedihydrofuran (DCDHF) class of single-molecule emitters that contain an amine electron donor and a,DCDHF acceptor linked by a conjugated unit; DCDHF-N-6 and DCDHF-A-6 have naphthalene- and anthracene-conjugated linkers, respectively. These molecules maintain the beneficial photophysics of the phenylene-linked DCDHF (i.e., photostability, emission wavelength dependence on solvent polarity, and quantum yield sensitivity to solvent viscosity), yet offer absorption and emission at longer wavelengths that are more appropriate for cellular imaging. We demonstrate that these new fluorophores are less photolabile in an aqueous environment than several other commonly used dyes (rhodamine 6G, Texas Red, and fluorescein). Finally, we image single copies of the acene DCDHFs diffusing in the plasma membrane of living cells.