Ciprofloxacin enhances stress erythropoiesis in spleen and increases survival after whole-body irradiation combined with skin-wound trauma.
Ciprofloxacin enhances stress erythropoiesis in spleen and increases survival after whole-body irradiation combined with skin-wound trauma.
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DOI：
10.1371/journal.pone.0090448
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kiang JG
中科院分区：
文献类型：
--
作者：
Fukumoto R;Burns TM;Kiang JG
严重的造血功能丧失是放射复合伤(CI)后的主要治疗靶点之一。在这项研究中，我们测试了环丙沙星(CIP)作为一种治疗方法的使用，因为最近报道的针对CI的免疫调节作用可能会改善造血。CIP方案为每日1次口服，连续3周，首次给药时间为脑梗塞后2小时。CIP治疗提高了小鼠30天的存活率，与未经治疗的对照组的35%相比，提高了80%。对早期血液学参数变化的研究发现，CI诱导的进展性贫血持续10天，CIP显著改善。CI诱导肾脏促红细胞生成素(EPO)基因表达，肾脏和血清蛋白表达，CIP刺激EPO基因表达。在CI小鼠的脾中，CIP通过EPO受体诱导巨噬细胞表达骨形态发生蛋白4(BMP4)。经CIP处理的CI小鼠脾细胞形成CD71+集落形成单位红系明显好于对照组。因此，CIP介导的BMP4依赖的应激性红细胞生成可能在提高脑梗塞后的存活率中发挥作用。
Severe hematopoietic loss is one of the major therapeutic targets after radiation-combined injury (CI), a kind of injury resulting from radiation exposure combined with other traumas. In this study, we tested the use of ciprofloxacin (CIP) as a treatment, because of recently reported immunomodulatory effects against CI that may improve hematopoiesis. The CIP regimen was a daily, oral dose for 3 weeks, with the first dose 2 h after CI. CIP treatment improved 30-day survival in mice at 80% compared to 35% for untreated controls. Study of early changes in hematological parameters identified CI-induced progressive anemia by 10 days that CIP significantly ameliorated. CI induced erythropoietin (EPO) mRNA in kidney and protein in kidney and serum; CIP stimulated EPO mRNA expression. In spleens of CI mice, CIP induced bone morphogenetic protein 4 (BMP4) in macrophages with EPO receptors. Splenocytes from CIP-treated CI mice formed CD71+ colony-forming unit-erythroid significantly better than those from controls. Thus, CIP-mediated BMP4-dependent stress erythropoiesis may play a role in improving survival after CI.
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影响因子：
3.7
作者：
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