Toluidine blue staining identifies high-risk primary oral premalignant lesions with poor outcome
Toluidine blue staining identifies high-risk primary oral premalignant lesions with poor outcome
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DOI：
10.1158/0008-5472.can-04-3153
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发表时间：
2005-09-01
期刊：
影响因子：
11.2
通讯作者：
Rosin, MP
中科院分区：
文献类型：
--
作者：
Zhang, LW;Williams, M;Rosin, MP
有一个迫切需要的发展，这将有助于检测口腔癌前病变（OPLs）的进展风险高的视觉辅助工具。初步数据表明，甲苯胺蓝染色可能优先保留的OPL与高风险的分子克隆。在这项研究中，我们监测了100例没有任何口腔癌病史的患者的OPL，平均44个月，以评估甲苯胺蓝状态与临床病理风险因素、分子模式（7个染色体臂的微卫星分析：3p、9p、4q、Sp、11q、13q和17p）和结果的相关性。甲苯胺蓝阳性染色与临床病理危险因素和高危分子危险模式相关。值得注意的是，甲苯胺蓝阳性病变观察到癌症风险增加> 6倍，在15个病变中有12个病变出现阳性染料滞留，随后进展为癌症（P = 0.0008）。甲苯胺蓝状态与危险因素和预后的相关性是明显的，即使分析仅限于低度异型增生或无异型增生的OPL。我们的研究结果表明，甲苯胺蓝在确定高风险OPL的潜在用途。
There is a pressing need for the development of visual aids that will facilitate the detection of oral premalignant lesions (OPLs) with a high-risk of progression. Preliminary data suggest that toluidine blue stain may be preferentially retained by OPLs with high-risk molecular clones. In this study, we monitored OPLs from 100 patients without any history of oral cancer for an average of 44 months in order to evaluate the association of toluidine blue status with clinicopathologic risk factors, molecular patterns (microsatellite analysis on seven chromosome arms: 3p, 9p, 4q, Sp, 11q, 13q, and 17p) and outcome. Toluidine blue-positive staining correlated with clinicopathologic risk factors and high-risk molecular risk patterns. Significantly, a > 6-fold elevation in cancer risk was observed for toluidine bluepositive lesions, with positive retention of the dye present in 12 of the 15 lesions that later progressed to cancer (P = 0.0008). This association of toluidine blue status with risk factors and outcome was evident even when the analysis was restricted to OPLs with low-grade or no dysplasia. Our results suggest the potential use of toluidine blue in identifying high-risk OPLs.