Two-channel, quasi-confocal parallel scan fluorescence imaging for detection of biochips
Two-channel, quasi-confocal parallel scan fluorescence imaging for detection of biochips
复制标题
用于检测生物芯片的双通道准共焦并行扫描荧光成像
DOI：
10.1016/j.optlaseng.2010.04.006
复制
发表时间：
2010-09-01
影响因子：
4.6
通讯作者：
Guo, Jihua
中科院分区：
文献类型：
--
作者：
Liu, Zhiyi;He, Yonghong;Guo, Jihua
近年来，生物芯片在生物医学应用方面取得了巨大进展。新的荧光成像方法已被开发用于生物芯片分析。本文提出了一种双通道、准共焦并行扫描荧光成像方法，用于生物芯片的检测。开发了一种成像系统，其具有一维光学扫描以产生二维宽视场图像，并且该系统被设计成能够简单地交换覆盖不同光谱区域的激光。准共聚焦成像的机制提供了每平方微米0.46个荧光分子的灵敏度，而平行扫描使其成为用于快速检测生物芯片的高通量技术。(c)2010爱思唯尔有限公司版权所有。
Biochips have advanced tremendously for biomedical applications in recent years. New fluorescence imaging methods have been developed for biochip analysis. In this work, a two-channel, quasi-confocal parallel scan fluorescence imaging method is proposed for detection of biochips. An imaging system was developed with one-dimensional optical scan to produce two-dimensional wide-field images, and the system was designed to enable simple exchange of lasers covering different spectral regions. The mechanism of quasi-confocal imaging provides a sensitivity of 0.46 fluorescence molecules per square micron, while parallel scan makes it a high throughput technique for rapid detection of biochips. (c) 2010 Elsevier Ltd. All rights reserved.