Ultrastructural localization of argyrophilic substances in diffuse plaques of Alzheimer-type dementia demonstrated by methenamine silver staining
Ultrastructural localization of argyrophilic substances in diffuse plaques of Alzheimer-type dementia demonstrated by methenamine silver staining
复制标题
乌洛托品银染色显示阿尔茨海默型痴呆弥漫斑块中嗜银物质的超微结构定位
DOI：
10.1007/bf00310135
复制
发表时间：
2004
影响因子：
12.7
通讯作者：
S. Hirai
中科院分区：
文献类型：
--
作者：
T. Yamazaki;H. Yamaguchi;K. Okamoto;S. Hirai
应用六亚甲基四胺银（MS）电子显微镜预包埋法观察了阿尔茨海默型痴呆脑弥漫斑嗜银物质的超微结构。弥漫性斑块中弥散分布着电子致密物质，这些物质是带有银颗粒的条纹状结构的稀疏聚集体。连续MS和常规MRI切片的比较显示，弥漫性斑块通常在模糊的细胞突起膜之间显示分散的淀粉样纤维束或无定形物质，或两者兼而有之。这些过程中的一些被确定为星形胶质细胞或树突状起源。在大的银阳性区域中经常观察到一些退行性神经突，但在小区域中从未观察到。这些发现表明，在某些细胞突起之间出现淀粉样纤维和无定形物质是老年斑形成过程中的一种非常形态学的变化。
The ultrastructure of argyrophilic substances in diffuse plaques of an Alzheimer-type dementia brain was examined using methenamine silver (MS) electron microscopy with the pre-embedding method. Electrondense substances, which were sparse aggregations of bundle-like structures with silver granules, were disseminated in the diffuse plaques. Comparison of serial MS and routine ultrathin sections revealed that diffuse plaques usually show scattered bundles of amyloid fibrils or amorphous materials, or both, between indistinct cell process membranes. Some of these processes were identified as astrocytic or dendritic in origin. A few degenerative neurites were frequently noted in large silver-positive areas but never in small areas. These findings suggest that the appearance of amyloid fibrils and amorphous materials between certain cell processes is a very morphological change in the process of senile plaque formation.