Identification of regions responsible for the function of the plant K+ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes
Identification of regions responsible for the function of the plant K+ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes
复制标题
酿酒酵母和非洲爪蟾卵母细胞中负责植物 K 通道 KAT1 和 AKT2 功能的区域的鉴定
DOI：
10.1080/19336950.2017.1372066
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发表时间：
2017
期刊：
影响因子：
3.3
通讯作者：
Uozumi Nobuyuki
中科院分区：
文献类型：
--
作者：
Saito Shunya;Hoshi Naomi;Zulkifli Lalu;Widyastuti Sri;Goshima Shinobu;Dreyer Ingo;Uozumi Nobuyuki
拟南芥K+通道KAT1在K+限制的培养基中补充K+摄取缺陷酿酒酵母突变株CY162的生长，而另一种K+通道AKT2则不能。为了深入了解这种差异的结构基础，我们用这两个基因构建了12个重组嵌合通道。当这些嵌合体在Cy162中表达时，只有3个嵌合体在K+限制的条件下完全挽救了Cy162的生长。我们认为KAT1的跨膜核心区对其INS活性具有重要作用。酿酒。这不仅涉及到孔洞区域，而且还涉及到其电压传感器区域的一部分。
TheArabidopsisK+channel KAT1 complements in K+-limited medium the growth of the K+uptake defectiveSaccharomyces cerevisiaemutant strain CY162, while another K+channel, AKT2, does not. To gain insight into the structural basis for this difference, we constructed 12 recombinant chimeric channels from these two genes. When expressed in CY162, only three of these chimeras fully rescued the growth of CY162 under K+-limited conditions. We conclude that the transmembrane core region of KAT1 is important for its activity inS. cerevisiae. This involves not only the pore region but also parts of its voltage-sensor domain.