Molecular aids for integration of alien chromatin through wide crosses
Molecular aids for integration of alien chromatin through wide crosses
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DOI：
10.1139/gen-42-4-584
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发表时间：
1999-08-01
期刊：
影响因子：
3.1
通讯作者：
Fedak, G
中科院分区：
文献类型：
--
作者：
Fedak, G
小麦广泛杂交至今已有100多年的历史。在这段时间里，大约有100个基因被转移到许多性状，包括生物和非生物胁迫和增值性状。外来来源的抗性基因确实会随着时间的推移而失效，因此必须继续寻找额外的可变性。最近对外来物种的筛选已经确定了具有多种抗虫性以及抗虫性和增值性状组合的品种。大多数现有的诱导重组体是非补偿型的，具有相当大的连锁阻力，因此建议顺序使用Ph突变体来产生较小的间隙重组体。包括GISH、RAPD、RFLP、AFLP和微卫星在内的分子方法被广泛用于鉴定完整的外来染色体、染色体片段和基因。
Wide crosses in wheat have now been performed for over 100 years. In that time, approximately 100 genes have been transferred for numerous traits, including biotic and abiotic stresses and value-added traits. Resistance genes from alien sources do become defeated with rime, so the search for additional variability must continue. Recent screening of alien species has identified accessions with multiple pest resistance plus combinations of pest resistance and value-added traits. The majority of existing induced recombinants are of a noncompensating type with considerable linkage drag, so sequential useage of Ph mutants is recommended to produce smaller interstitial recombinants. Molecular methods including GISH, RAPD, RFLP, AFLP, and microsatellites, are being widely used to identify integrated alien chromosomes, chromosome segments, and genes.