Evolutionary analysis of the ENTH/ANTH/VHS protein superfamily reveals a coevolution between membrane trafficking and metabolism
Evolutionary analysis of the ENTH/ANTH/VHS protein superfamily reveals a coevolution between membrane trafficking and metabolism
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DOI：
10.1186/1471-2164-13-297
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发表时间：
2012-07-02
期刊：
影响因子：
4.4
通讯作者：
Friant, Sylvie
中科院分区：
文献类型：
--
作者：
De Craene, Johan-Owen;Ripp, Raymond;Friant, Sylvie
背景：膜转运涉及蛋白质和脂类在细胞内定位的复杂调节，是代谢摄取、细胞生长和发育所必需的。通过内体的不同运输途径由Eth/Anth/VHS接头蛋白超家族协调。结果：我们对这个由84个代表不同真核生物类群的完整基因组中的蛋白质组成的超家族进行了计算机分析，发现这个超家族的基因组分布可以区分真菌和后生动物与植物和原生动物。接下来，在四个方向的全基因组比较中，我们发现这一区别特征不仅在其他膜转运效应因子中观察到，而且在参与代谢和胞质分裂的蛋白质中也观察到，这表明新陈代谢、胞质分裂和细胞内转运途径是共同进化的。此外，一些已鉴定的蛋白质涉及多种功能，要么参与运输和代谢，要么涉及运输和胞质分裂，这表明膜运输是这种共同进化过程的核心。结论：膜运输和区划不仅是真核细胞出现的关键特征，而且推动了真核生物在不同类群中的分离。
Background: Membrane trafficking involves the complex regulation of proteins and lipids intracellular localization and is required for metabolic uptake, cell growth and development. Different trafficking pathways passing through the endosomes are coordinated by the ENTH/ANTH/VHS adaptor protein superfamily. The endosomes are crucial for eukaryotes since the acquisition of the endomembrane system was a central process in eukaryogenesis.Results: Our in silico analysis of this ENTH/ANTH/VHS superfamily, consisting of proteins gathered from 84 complete genomes representative of the different eukaryotic taxa, revealed that genomic distribution of this superfamily allows to discriminate Fungi and Metazoa from Plantae and Protists. Next, in a four way genome wide comparison, we showed that this discriminative feature is observed not only for other membrane trafficking effectors, but also for proteins involved in metabolism and in cytokinesis, suggesting that metabolism, cytokinesis and intracellular trafficking pathways co-evolved. Moreover, some of the proteins identified were implicated in multiple functions, in either trafficking and metabolism or trafficking and cytokinesis, suggesting that membrane trafficking is central to this co-evolution process.Conclusions: Our study suggests that membrane trafficking and compartmentalization were not only key features for the emergence of eukaryotic cells but also drove the separation of the eukaryotes in the different taxa.