Effect of a graded single constriction of the rat sciatic nerve on pain behavior and expression of immunoreactive NPY and NPYY1 receptor in DRG neurons and spinal cord
Effect of a graded single constriction of the rat sciatic nerve on pain behavior and expression of immunoreactive NPY and NPYY1 receptor in DRG neurons and spinal cord
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DOI：
10.1016/j.brainres.2003.09.085
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发表时间：
2004-04-23
期刊：
影响因子：
2.9
通讯作者：
Villar, MJ
中科院分区：
文献类型：
--
作者：
Brumovsky, PR;Bergman, E;Villar, MJ
在本研究中，仅使用一根结扎线，在神经和结扎线之间放置一个非常薄的聚乙烯鞘，使大鼠坐骨神经不同程度地收缩。完全神经横断进行了研究比较。在神经收缩40-80%的情况下，我们观察到异常性疼痛的程度与基于四条松散结扎线的所谓班尼特模型相似。我们还监测了神经肽Y（NPY）和NPY Y1受体（Y1 R）在腰4-5背根神经节（DRG）和背角的表达变化，发现损伤后DRG神经元中NPY上调和Y1 R下调。这些结果表明，类似的肽和受体的变化发生在这个模型后轴突切断和其他神经损伤模型，虽然免疫组织化学和行为的变化似乎是依赖于神经的收缩程度。因此，在评估疼痛和其他损伤后事件时，监测收缩程度似乎是相关的。的可能性，在NPY能神经传递的一些变化有关的产生异常性疼痛进行了讨论，以及使用这种mononeuropathic模型的基础上，一个单一的结扎神经收缩（SLNC）作为一种补充方法，以其他广泛使用的疼痛模型的可能性。(C)2004 Elsevier B. V.保留所有权利。
In the present study, the rat sciatic nerve was constricted to varying degrees using only one ligature with a very thin polyethylene sheath placed between nerve and ligature thread. Complete nerve transection was studied for comparison. With a 40-80% constriction of the nerve we observed allodynia to a similar extent as in the so-called Bennett model based on four loose ligatures. We also monitored changes in the expression of neuropeptide Y (NPY) and the NPY Y1 receptor (Y1R) in the lumbar 4-5 dorsal root ganglia (DRG) and dorsal horn and found upregulation of NPY and downregulation of the Y1R in DRG neurons after injury. These results indicate that similar peptide and receptor changes occur in this model as after axotomy and in other nerve injury models, although the immunohistochemical and behavioral changes seem to be dependent on the degree of constriction of the nerve. Thus, it seems relevant to monitor the degree of constriction when evaluating pain and other post-injury events. The possibility that some of the changes in NPY-ergic neurotransmission are related to the generation of allodynia is discussed; as well as the possibility to use this mononeuropathic model based on a single ligature nerve constriction (SLNC) as a complementary approach to other widely used pain models. (C) 2004 Elsevier B.V. All rights reserved.