CHANGES IN THE ARRANGEMENT OF MICROTUBULES AND MICROFIBRILS IN DIFFERENTIATING CONIFER TRACHEIDS DURING THE EXPANSION OF CELLS
CHANGES IN THE ARRANGEMENT OF MICROTUBULES AND MICROFIBRILS IN DIFFERENTIATING CONIFER TRACHEIDS DURING THE EXPANSION OF CELLS
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DOI：
10.1006/anbo.1995.1025
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发表时间：
1995-03-01
期刊：
影响因子：
4.2
通讯作者：
FUKAZAWA, K
中科院分区：
文献类型：
--
作者：
ABE, H;FUNADA, R;FUKAZAWA, K
采用免疫荧光和场发射扫描电镜观察了萨沙林冷杉管胞在细胞扩增过程中微管和径向壁纤维素微原纤维的排列。管胞的径向直径增加到形成层初始细胞的3 ~ 4倍。早期针叶树管胞初生壁最内层表面的微原纤维排列不整齐，大部分微原纤维呈纵向排列。随着细胞的扩大，微原纤维在沉积过程中仍然排列不整齐，但其取向由纵向向横向转变。当细胞停止扩张时，微原纤维排列有序，横向定向。皮层微管的取向变化与微原纤维相似。这些结果表明，针叶树管胞皮层微管的方向与微原纤维的方向和细胞形态发生有关。
The arrangements of microtubules and the cellulose microfibrils of radial walls in tracheids of Abies sachalinensis Masters during the expansion of cells were examined by immunofluorescence and field-emission scanning electron microscopy. The radial diameter of tracheids increased to three to four times that of cambial initial cells. Microfibrils on the innermost surface of primary walls of conifer tracheids at early stages were not well ordered and most of the microfibrils were oriented longitudinally. As each cell expanded, microfibrils in the process of deposition were still not well ordered but their orientation changed from longitudinal to transverse. When cell expansion ceased, microfibrils were well ordered and oriented transversely. Cortical microtubules showed a change in orientation similar to that of the microfibrils. These results indicate that the orientation of cortical microtubules is correlated with that of microfibrils as they are being laid down and with cell morphogenesis in conifer tracheids.