Liposome-Mediated DNA Transfer in Eukaryotic Cells: Gene Uptake and Expression in the Host Cell
Liposome-Mediated DNA Transfer in Eukaryotic Cells: Gene Uptake and Expression in the Host Cell
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真核细胞中脂质体介导的 DNA 转移：宿主细胞中的基因摄取和表达
DOI：
10.1007/978-1-4684-4241-0_23
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
C. Sené
中科院分区：
文献类型：
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作者：
C. Nicolau;C. Sené
脂质体已用于引入药物（Poste和Papahadjopoulos，1976）、酶（Gregoriadis和巴克兰，1973）、mRNA（Dimitriadis，1978）、病毒（Lonberg-Holm等人，1976; Wilson等人，1977）和变构效应物（Nicolau和Gersonde，1979; Gersonde和Nicolau，1979）。最近，染色体已经被捕获在脂质体中并转移到细胞中（Mukherjee等人，1978）并且DNA已经被引入细菌细胞（Fraley等人，1979）和植物细胞（Lurquin，1979）。四环素抗性基因TetR在大肠杆菌中也有表达。在脂质体介导的质粒pBR 322转移到这些细胞中后，将其转化到大肠杆菌中（Fraley等，1979年）。Fraley等（1981）使用脂质体包封从SV 40病毒分离的高效DNA。用这些装载DNA的脂质体感染允许的猴细胞系导致脂质体中包封的SV 40 DNA的表达，其效率与磷酸钙方法相当。Wong等（1980）从E.大肠杆菌（coli）中获得了编码β-内酰胺酶活性的875 bp的限制性内切酶片段，并将其包埋在脂质体中。将载有DNA的脂质体与禽类、鼠和人细胞一起孵育，导致细胞摄取DNA并表达β-内酰胺酶活性，如通过光谱和微生物学方法测定的。最近，Schaid-Ridder等（1981）用脂质体包封的胸苷激酶基因转化小鼠LMTK细胞，Nicolau和Rottem（1981）转化M. caprilavine与包封在脂质体中的pBR 322质粒的结合，从而赋予该四环素敏感生物体四环素抗性。我们将在这里讨论用脂质体包裹的遗传物质转化真核细胞的几个方面。
Liposomes have been used to introduce drugs (Poste and Papahadjopoulos, 1976), enzymes (Gregoriadis and Buckland, 1973), mRNA (Dimitriadis, 1978), viruses (Lonberg-Holm et al., 1976; Wilson et al., 1977), and allosteric effectors (Nicolau and Gersonde, 1979; Gersonde and Nicolau, 1979) in a number of different cells. Recently, chromosomes have been entrapped in liposomes and transferred to cells (Mukherjee et al., 1978) and DNA has been introduced into bacterial cells (Fraley et al., 1979) and into plant cells (Lurquin, 1979). Also the expression of the tetracycline resistance gene (TetR) has been observed in E. coli after liposome-mediated transfer of the plasmid pBR322 to these cells (Fraley et al., 1979). Fraley et al. (1981) have used liposomes to encapsulate with high efficiency DNA isolated from SV40 virus. Infection of a permissive monkey cell line with these DNA-loaded liposomes resulted in the expression of the SV40 DNA encapsulated in liposomes with efficiencies comparable to the calcium phosphate method. Wong et al. (1980) isolated, from E. coli a restriction fragment, 875 bp, which encodes for a β-lactamase activity, and entrapped it in liposomes. Incubation of the DNA-loaded liposomes with avian, murine and human cells resulted in the uptake by the cells of the DNA and the expression of a β-lactamase activity as determined by spectroscopic and microbiological methods. Recently, Schaeffer-Ridder et al. (1981) transformed mouse LMTK cells with the liposome-encapsulated thymidine kinase gene and Nicolau and Rottem (1981) transformed M. capriolum with the pBR322 plasmid encapsulated in liposomes, conferring thus tetracycline resistance to this tetracycline sensitive organism. We will discuss here several aspects of the transformation of eukaryotic cells with liposome encapsulated genetic material.