Inflammatory response during the muscle phase of Trichinella spirals and T-pseudospiralis infections
Inflammatory response during the muscle phase of Trichinella spirals and T-pseudospiralis infections
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DOI：
10.1007/s004360100420
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发表时间：
2001-09-01
影响因子：
2
通讯作者：
Ko, RC
中科院分区：
文献类型：
--
作者：
Li, CKF;Ko, RC
试图确定是否同时感染旋毛虫和旋毛虫。pseudospiralis可以减少蠕虫发育的肌肉阶段期间针对前一物种的细胞浸润。BALB/c、裸鼠和CBA/N小鼠经口感染任一种或两者的混合物。还将任一种或两种物种的新生幼虫（NBL）皮下注射到BALB/c小鼠的右/左腿中。于T. BALB/c小鼠感染旋毛虫后，肌炎症状最重，CBA/N次之，裸鼠最轻。于T.在BALB/c小鼠中，蠕虫周围有轻微的细胞浸润，而在裸鼠或CBA/N小鼠中则无。然而，在两种口腔混合感染中，T.螺旋体未缩小。在注射T. pseudospiralis NBL，感染期幼虫周围的浸润大多不存在。然而，在注射T. spiralis NBL，在蠕虫部位附近观察到明显的肉芽肿反应。组织反应明显强于口腔感染。在注射两种物种的NBL的小鼠中（注射到不同的腿中），在T.螺旋形的BALB/c小鼠注射活的或死的T淋巴细胞后，其淋巴细胞中IL-4和IL-6水平均显著升高。在注射后第15天和第20天，这表明蠕虫主要在肌肉发育阶段引起T（H）2反应。间接荧光抗体试验和激光共聚焦显微镜研究表明，在T.螺旋形的
An attempt was undertaken to determine whether a concurrent infection of Trichinella spiralis and T. pseudospiralis can reduce cellular infiltrations against the former species during the muscle phase of worm development. BALB/c, nude and CBA/N mice were orally infected with either species or a mixture of both. New-born larvae (NBL) of either or both species were also injected subcutaneously into the right/left leg of BALB/c mice. In T. spiralis oral infection, myositis was strongest in BALB/c, intermediate in CBA/N and weakest in nude mice. In T. pseudospiralis oral infection, slight cellular infiltrations were observed around the worms in BALB/c but not in nude or CBA/N mice. However, in mixed oral infections of two species, infiltrations around the sites of T. spiralis were not reduced. In mice injected with T. pseudospiralis NBL, infiltrations around the infective-stage larvae were mostly absent. However, in mice injected with T. spiralis NBL, prominent granulomatous reactions were observed near the sites of worms. The tissue reaction was substantially stronger than that in oral infections. In mice injected with NBL of both species (into different legs), a heavy infiltration was also observed at the site of T. spiralis. A marked increase in levels of IL-4 and IL-6 was detected in the popliteal lymphocytes of BALB/c mice injected with either live or dead NBL of T. spiralis at days 15 and 20 post-injection. This indicated that the worms mainly elicited a T(H)2 response during the muscle phase of development. An indirect fluorescent antibody test and laser confocal microscopic studies demonstrated the presence of CD4 and CD8 cells in the cytoplasmic region of the nurse cell complex of T. spiralis.