Evidence that a second tumor antigen coded by adenovirus early gene region E1a is required for efficient cell transformation.
Evidence that a second tumor antigen coded by adenovirus early gene region E1a is required for efficient cell transformation.
复制标题
有证据表明，有效的细胞转化需要由腺病毒早期基因区域 E1a 编码的第二种肿瘤抗原。
DOI：
10.1073/pnas.82.1.163
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发表时间：
1985
影响因子：
11.1
通讯作者：
G. Chinnadurai
中科院分区：
文献类型：
--
作者：
D. Hurwitz;G. Chinnadurai
腺病毒（Ad）早期编码区1a（E1a）的表达对于病毒诱导的细胞转化以及其它病毒早期基因和一些细胞基因的激活是必需的。从该区域转录的13S和12S的两个重叠的早期mRNA分别编码289个氨基酸的蛋白质和243个氨基酸的蛋白质。早期的研究表明，289个氨基酸的蛋白质是细胞转化所必需的。我们已经构建了一个Ad 2型（Ad2）缺失突变体（dl231），其中13S mRNA的插入序列被精确去除。突变体dl231在人KB细胞中完全存活，并产生正常量的13S mRNA，但产生大量减少的缺陷12S mRNA。突变体dl231以野生型病毒的五分之一到一半的频率诱导已建立的大鼠胚胎成纤维细胞的局灶性转化。然而，转化细胞在半固体培养基上形成非贴壁依赖性集落的能力有缺陷。因此，我们的研究结果表明，243个氨基酸的蛋白质是完全转化大鼠胚胎细胞所必需的。
The expression of the adenovirus (Ad) early coding region 1a (E1a) is required for virus-induced cell transformation and for the activation of other viral early genes and some cellular genes. Two overlapping early mRNAs of 13S and 12S that are transcribed from this region code for a 289-amino acid protein and a 243-amino acid protein, respectively. Earlier studies have shown that the 289-amino acid protein is essential for cell transformation. We have constructed an Ad type 2 (Ad2) deletion mutant (dl231) in which the intervening sequence for the 13S mRNA is precisely removed. Mutant dl231 is completely viable in human KB cells and produces normal amounts of 13S mRNA but much reduced amounts of a defective 12S mRNA. Mutant dl231 induces focal transformation of established rat embryo fibroblasts at a frequency one-fifth to one-half that of wild-type virus. However, the transformed cells are defective in their ability to form anchorage-independent colonies on semisolid medium. Therefore, our results demonstrate that the 243-amino acid protein is required for full transformation of rat embryo cells.