PERIPHERAL ORIGINS AND CENTRAL MODULATION OF SUBCUTANEOUS FORMALIN-INDUCED ACTIVITY OF RAT DORSAL HORN NEURONS
PERIPHERAL ORIGINS AND CENTRAL MODULATION OF SUBCUTANEOUS FORMALIN-INDUCED ACTIVITY OF RAT DORSAL HORN NEURONS
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DOI：
10.1016/0304-3940(87)90242-4
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发表时间：
1987-12-16
影响因子：
2.5
通讯作者：
SULLIVAN, AF
中科院分区：
文献类型：
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作者：
DICKENSON, AH;SULLIVAN, AF
在脊髓区域L1-L3中的单个会聚背角神经元的细胞外记录在氟烷麻醉的大鼠中进行，所述氟烷在N2 O和O2的气体混合物中。先前已经发现皮下福尔马林（5%，50 μ l）在相同的制备物中产生背角会聚神经元的延长的明显的双相反应。本研究表明，在约20分钟时发生并持续至少另外40分钟的第二神经元兴奋期可以被注射到福尔马林注射部位的木质素（2%，50 μ l）消除（n=5）。福尔马林诱发的活动的抑制持续约10-20分钟，然后由神经元反应的完全恢复。相同剂量的i. v.木质素对福尔马林诱导的神经元活性没有影响（n=4）。深刻的抑制的第二阶段也产生了触觉节段刺激和伤害性刺激施加到身体的广泛领域（弥漫性伤害性抑制控制）。这些发现是关于长时间伤害性刺激的外周和中枢后果进行讨论的。
Extracellular recordings of single convergent dorsal horn neurones in the spinal cord region L1-L3 were made in rats anesthetised with halothane in a gaseous mix of N2O and O2. Subcutaneous formalin (5%, 50 .mu.l) has previously been found to produce a prolonged distinct biphasic response of dorsal horn convergent neurones in the same preparation. The present study demonstrates that the second period of neuronal excitation which occurs at about 20 min and lasts for at least another 40 min, could be abolished by lignocaine (2%, 50 .mu.l) injected into the site of the formalin injection (n=5). The inhibition of formalin-evoked activity lasted for about 10-20 min and was followed by complete recovery of the neuronal response. The same dose of i.v. lignocaine had no effect on the formalin-induced neuronal activity (n=4). Profound inhibitions of the second phase were also produced by tactile segmental stimulation and noxious stimuli applied to widespread areas of the body (diffuse noxious inhibitory controls). These findings are discussed with regard to the peripheral and central consequences of prolonged noxious stimuli.