DNA Polymer Brush Patterning through Photocontrollable Surface-Initiated DNA Hybridization Chain Reaction.
DNA Polymer Brush Patterning through Photocontrollable Surface-Initiated DNA Hybridization Chain Reaction.
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通过光控表面引发的 DNA 杂交链式反应进行 DNA 聚合物刷图案化
DOI：
10.1002/smll.201501826
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发表时间：
2015
期刊：
影响因子：
13.3
通讯作者：
Liang Haojun
中科院分区：
文献类型：
--
作者：
Huang Fujian;Zhou Xiang;Yao Dongbao;Xiao Shiyan;Liang Haojun
在固体表面制备具有空间分辨率的DNA聚合物刷子是生物芯片研究及相关应用、无细胞基因表达研究甚至人造细胞制造的关键一步。在这里，首次报道了一种DNA聚合物刷图案化方法，该方法基于嵌入邻硝基苄基连接物的DNA发夹结构的光激活和随后的表面引发的DNA杂交链反应(HCR)。惰性DNA发夹暴露在紫外光照射下，以可编程的方式产生具有两个活性粘性末端(脚趾)的DNA双链。这些被激活的DNA双链可以引发DNA HCR产生具有复杂几何形状的多功能图案化DNA聚合物刷子。利用这种方法，可以在同一固体表面的特定区域上制作不同的多功能DNA聚合物刷子图案。此外，图案化的DNA刷面可以用来以所需的方式捕获目标分子。
The fabrication of DNA polymer brushes with spatial resolution onto a solid surface is a crucial step for biochip research and related applications, cell‐free gene expression study, and even artificial cell fabrication. Here, for the first time, a DNA polymer brush patterning method is reported based on the photoactivation of an ortho‐nitrobenzyl linker‐embedded DNA hairpin structure and a subsequent surface‐initiated DNA hybridization chain reaction (HCR). Inert DNA hairpins are exposed to ultraviolet light irradiation to generate DNA duplexes with two active sticky ends (toeholds) in a programmable manner. These activated DNA duplexes can initiate DNA HCR to generate multifunctional patterned DNA polymer brushes with complex geometrical shapes. Different multifunctional DNA polymer brush patterns can be fabricated on certain areas of the same solid surface using this method. Moreover, the patterned DNA brush surface can be used to capture target molecules in a desired manner.