PREPARATION CONDITIONS AND EVALUATION OF THE STABILITY OF LIPID VESICLES (LIPOSOMES) USING THE MICROENCAPSULATION TECHNIQUE
PREPARATION CONDITIONS AND EVALUATION OF THE STABILITY OF LIPID VESICLES (LIPOSOMES) USING THE MICROENCAPSULATION TECHNIQUE
复制标题
微囊化技术脂质囊泡（脂质体）的制备条件及稳定性评价
DOI：
10.1080/01932698808943973
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发表时间：
1988
影响因子：
2.2
通讯作者：
H. Ogata
中科院分区：
文献类型：
--
作者：
F. Ishii;A. Takamura;H. Ogata
摘要详细介绍了微胶囊化技术中的水干燥法制备脂质囊泡（脂质体）的条件。该过程包括以下步骤：（I）将待包封的药物的水溶液分散到有机溶剂中以产生油包水乳液，（II）将该w/o型乳液分散到水相中（蒸馏水），（III）通过从油相中蒸发有机溶剂在液滴表面上形成脂质膜，和（IV）通过超离心或通过Sephadex柱的凝胶过滤除去游离标记物（药物）。在该过程中，囊泡的平均直径取决于将w/o型乳液分散到蒸馏水中所需的机械转数。此外，脂质囊泡的包封率随着将w/o型乳液分散到蒸馏器中所需的机械转数的降低而增加。
Abstract The conditions for the preparation of lipid vesicles ((liposomes) by the in-water drying method, one of the microencapsulation techniques, were described in detail. This process consists of the following steps: (I) dispersion of an aqueous solution of the drug to be encapsulated into an organic solvent to yield a water-in-oil emulsion, (II) dispersion of this w/o-type emulsion into an aqueous phase (distilled water), (III) formation of a lipid membrane on the surface of droplets by evaporation of the organic solvent from the oil phase, and (IV) removal of the free marker (drug) by ultracentrifugation or gel filtration through a Sephadex column. In this process, the mean diameter of the vesicles was dependent on the mechanical revolution number necessary for dispersing the w/o-type emulsion into the distilled water. Moreover, the percentage encapsulation of the lipid vesicles increased with the decrease in mechanical revolution number necessary for dispersing the w/o-type emulsion into the distill...