VIRUS SPREAD AND INITIAL PATHOLOGICAL-CHANGES IN THE NERVOUS-SYSTEM IN GENITAL HERPES-SIMPLEX VIRUS TYPE-2 INFECTION IN MICE - A CORRELATIVE IMMUNOHISTOCHEMICAL, LIGHT AND ELECTRON-MICROSCOPIC STUDY
VIRUS SPREAD AND INITIAL PATHOLOGICAL-CHANGES IN THE NERVOUS-SYSTEM IN GENITAL HERPES-SIMPLEX VIRUS TYPE-2 INFECTION IN MICE - A CORRELATIVE IMMUNOHISTOCHEMICAL, LIGHT AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1007/bf00687270
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发表时间：
1987-01-01
影响因子：
12.7
通讯作者：
WEBSTER, HD
中科院分区：
文献类型：
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作者：
GEORGSSON, G;MARTIN, JR;WEBSTER, HD
小鼠通过阴道途径感染 2 型单纯疱疹病毒 (HSV-2) 的 MS 株。使用连续阴道培养物来确认感染并选择小鼠用于本研究。感染后第2-6天(p.i.)通过灌注杀死两只小鼠，固定并包埋腰椎和骶管尾部用于电子显微镜检查。使用兔抗 HSV-2 抗血清和亲和素-生物素 (ABC) 方法对半薄 Epon 切片进行病毒抗原染色。通过电子显微镜检查抗原阳性块的薄切片，并比较这两种方法检测到的感染细胞的数量和类型。通过这些方法检测感染细胞之间发现了良好的相关性。感染细胞包括背根神经节和脊髓的神经元、背根神经节的卫星细胞、非髓鞘雪旺细胞、星形胶质细胞、少突胶质细胞和蛛网膜细胞。感染细胞在注射后第 3 天首次在尾部检测到。注射后第 5 天在脊髓中受感染细胞的时间和空间分布与中枢神经系统和中枢神经系统内的神经扩散一致。病理病变与感染细胞的分布和数量具有良好的相关性，可能是由于直接的病毒效应所致。 Epon-ABC 方法在检测感染细胞时与电子显微镜相似的敏感性表明该方法可能在实验和诊断工作中具有有用的应用。
Mice were infected by the vaginal route with the MS strain of herpes simplex virus type 2 (HSV-2). Serial vaginal cultures were used to confirm infection and to select mice for this study. Two mice were killed by perfusion on days 2-6 post infection (p.i.) and lumbar and sacral cord with cauda were fixed and embedded for electron microscopy. Semithin Epon-sections were stained for viral antigen using a rabbit anti-HSV-2 antiserum and the Avidin-Biotin (ABC) method. Thin sections from antigen-positive blocks were examined by electron microscopy, and the number and types of infected cells detected by these two methods were compared. A good correlation was found between detection of infected cells by these methods. Infected cells included neurons of dorsal root ganglia and spinal cord, satellite cells of dorsal root ganglia, non-myelinating Schwann cells, astrocytes, oligodendrocytes and arachnoidal cells. Infected cells were first detected in the cauda on day 3 p.i. and in the spinal cord on day 5 p.i. The temporal and spatial distribution of infected cells was consistent with neural spread to and within the CNS. The pathological lesions showed a good correlation with the distribution and number of infected cells and are probably due to a direct virus effect. The similar sensitivity of the Epon-ABC method to electron microscopy in detecting infected cells indicates that this method may have useful applications in both experimental and diagnostic work.