Facile Quantification of Alzheimer’s Disease Amyloid-β Based On Aggregation-Induced Emission
Facile Quantification of Alzheimer’s Disease Amyloid-β Based On Aggregation-Induced Emission
复制标题
基于聚集诱导发射的阿尔茨海默氏病淀粉样蛋白-β 的简便定量
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
T. Murashima
中科院分区：
文献类型：
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作者：
Koji Kawamura;R. Nakayama;A. Matsumoto;S. Fujii;T. Murashima
基于聚合诱导发射（aggregation-induced emission，AIE）染料和修饰的淀粉样蛋白促凝血肽（amyloid firidization-promoting peptide，AFPP）探针，建立了一种简便的淀粉样蛋白β（amyloid beta，Aβ）检测新方法。探针的AFPP部分在加速Aβ纤维化中起作用，探针的AIE部分在信号发射中起作用。由于探针分子均匀分散在不含Aβ的缓冲溶液中，荧光强度较弱。在探针溶液中加入Aβ后，Aβ聚集加快，荧光强度在3 h内增强。该方法的检测限为4.2 nM。检测限值不足以定量体液中发现的Aβ;然而，本方法具有简单的“添加和测量”程序的优点，而不需要高水平的实验室技能和复杂的设备。
A novel, facile amyloid beta (Aβ) peptide detection method was developed based on an aggregation-induced emission (AIE) dye using a modified amyloid fibrillization-promoting peptide (AFPP) probe. The AFPP portion of the probe plays a role in accelerating Aβ fibrillization, and the AIE portion of the probe plays a role in signal emission. Since the probe molecules were uniformly dispersed in buffer solution without Aβ, the fluorescent intensity was weak. While Aβ was added to the probe solution, the aggregation of Aβ was promoted and the fluorescence was enhanced within 3 hrs. The limit of detection with this method is 4.2 nM. The limit of detection value is insufficient for the quantification of Aβ found in bodily fluids; however, the present method has the advantage of a simple “add-and-measure” procedure without the need for a high level of laboratory skills and sophisticated equipment.
影响因子：
4.9
作者：
Mathis CA;Mason NS;Lopresti BJ;Klunk WE
通讯作者：
Klunk WE