Elevated serum and synovial fluid TNF-like ligand 1A (TL1A) is associated with autoantibody production in patients with rheumatoid arthritis
Elevated serum and synovial fluid TNF-like ligand 1A (TL1A) is associated with autoantibody production in patients with rheumatoid arthritis
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DOI：
10.3109/03009742.2012.727026
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发表时间：
2013-02
影响因子：
2.1
通讯作者：
Xueting Sun;Jing Zhao;Rui Liu;Rulin Jia;Lingyun Sun;Xiang-Yang Li;Z. Li
中科院分区：
文献类型：
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作者：
Xueting Sun;Jing Zhao;Rui Liu;Rulin Jia;Lingyun Sun;Xiang-Yang Li;Z. Li
目的：肿瘤坏死因子 (TNF) 样配体 1A (TL1A) 与类风湿性关节炎 (RA) 相关，但其与 RA 的临床相关性尚未完全阐明。 We analysed TL1A levels in the serum and synovial fluid (SF) of RA patients and investigated its clinical significance.方法：通过酶联免疫吸附试验（ELISA）测定 109 名 RA 患者、29 名骨关节炎（OA）患者和 126 名健康对照者的 TL1A 水平。 Anti-cyclic citrullinated peptide (anti-CCP) antibodies and rheumatoid factor immunoglobulin G (RF-IgG) were tested by ELISA.通过标准实验室技术测量 RF-IgM、抗角蛋白抗体 (AKA) 和抗核周因子 (APF) 抗体、红细胞沉降率 (ESR)、C 反应蛋白 (CRP) 和免疫球蛋白。 The associations between TL1A and the clinical and serological features of RA were analysed. Results: TL1A concentrations were significantly elevated in both serum and SF of RA patients compared with OA patients and healthy controls. TL1A levels in RA SF were significantly higher than those in matched serum. A positive correlation was found between SF and serum TL1A levels.血清 TL1A 浓度与 RA 特异性自身抗体相关，包括 RF（RF-IgG、RF-IgM）和抗瓜氨酸蛋白抗体。 Antibody production by peripheral blood mononuclear cells (PBMCs) from RA patients was elevated upon TL1A stimulation. However, there was no correlation between serum or SF TL1A levels and RA disease activity.结论：TL1A 水平在 RA 血清和 SF 中显着升高，并且与 RA 中自身抗体的产生呈正相关，但不能作为疾病活动标志物。 TL1A 促进 RA 患者的 PBMC 产生抗体。 The role of TL1A in the humoral autoimmune response may be important in the development of RA.
Objectives: Tumour necrosis factor (TNF)-like ligand 1A (TL1A) is involved in rheumatoid arthritis (RA) but its clinical relevance in RA has not been fully elucidated. We analysed TL1A levels in the serum and synovial fluid (SF) of RA patients and investigated its clinical significance. Methods: TL1A levels were measured by enzyme-linked immunosorbent assay (ELISA) in 109 RA patients, 29 patients with osteoarthritis (OA), and 126 healthy controls. Anti-cyclic citrullinated peptide (anti-CCP) antibodies and rheumatoid factor immunoglobulin G (RF-IgG) were tested by ELISA. RF-IgM, anti-keratin antibody (AKA), and anti-perinuclear factor (APF) antibodies, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and immunoglobulins were measured by standard laboratory techniques. The associations between TL1A and the clinical and serological features of RA were analysed. Results: TL1A concentrations were significantly elevated in both serum and SF of RA patients compared with OA patients and healthy controls. TL1A levels in RA SF were significantly higher than those in matched serum. A positive correlation was found between SF and serum TL1A levels. Serum TL1A concentrations were associated with RA-specific autoantibodies including RFs (RF-IgG, RF-IgM) and anti-citrullinated protein antibodies. Antibody production by peripheral blood mononuclear cells (PBMCs) from RA patients was elevated upon TL1A stimulation. However, there was no correlation between serum or SF TL1A levels and RA disease activity. Conclusions: TL1A levels are significantly elevated in RA serum and SF and positively correlated with autoantibody production in RA, but failed as a disease activity marker. TL1A promotes antibody production by PBMCs from RA patients. The role of TL1A in the humoral autoimmune response may be important in the development of RA.