Selective Alteration of the Root Morphology of Arabidopsis thaliana by Synthetic Anion Transporters (SATs)
Selective Alteration of the Root Morphology of Arabidopsis thaliana by Synthetic Anion Transporters (SATs)
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DOI：
10.9734/csji/2019/v27i430119
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发表时间：
2019-08
期刊：
影响因子：
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通讯作者：
Mohit B Patel;Evan C. Garrad;S. Korb;S. Negin;Michael R Gokel;S. Sedinkin;Shanheng Yin;G. Gokel
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文献类型：
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作者：
Mohit B Patel;Evan C. Garrad;S. Korb;S. Negin;Michael R Gokel;S. Sedinkin;Shanheng Yin;G. Gokel
目的：本研究的目的是确定两亲性七肽合成阴离子转运蛋白（SATs）家族是否以及在多大程度上影响拟南芥的生长或根形态。研究设计：拟南芥幼苗在PNS培养基中生长，不含或存在21种sat中的一种。学习地点和时间：密苏里大学圣路易斯分校化学与生物化学系，1 University Blvd。该研究于2017-2018年进行。方法：制备了21个形式为R2N-COCH2YCH2CO-(Aaa)3Pro(Aaa)3-O(CH2)6CH3的化合物。氨基酸包括Ala、Gly和Ser。R是正常的烷基，有6、10、12或18个碳。Y是亚甲基，氧，硫，或者不存在。在PNS培养基中加入不同浓度的SAT，每组21株，培养11天。观察和/或测量植株的整体生长和根系形态，并记录结果。结果：通过对GGGPSGS型多肽序列主根长度和侧根数的比较发现，无论丝氨酸是否受到t-丁基保护，侧根密度的变化都最大。根据~COCH2YCH2CO~链中Y的同一性，还观察到这些肽序列的差异。结论：七肽c端丝氨酸氧原子的存在与Cl¯相互作用，导致离子浓度的变化，主根长度和侧根密度的改变。
Aims: The aim of the study was to determine whether and to what extent any of a family of amphiphilic heptapeptide synthetic anion transporters (SATs) affected the growth or root morphology of Arabidopsis thaliana. Study Design: A. thaliana plants were grown from seedlings in PNS media in the absence or presence of one of 21 SATs. Place and Duration of Study: Departments of Chemistry & Biochemistry, University of Missouri – St. Louis, 1 University Blvd., St. Louis, MO 63121 U. S. A. The study was conducted 2017-2018. Methodology: Twenty one compounds of the form R2N-COCH2YCH2CO-(Aaa)3Pro(Aaa)3-O(CH2)6CH3 were prepared and studied. The amino acids included Ala, Gly, and Ser. R was normal alkyl having 6, 10, 12, or 18 carbons. Y was methylene, oxygen, sulfur, or absent. The PNS media was infused with various concentrations of the SAT and 21 plants in each group were allowed to grow for 11 days. Overall plant growth and root morphology were visualized and/or measured and the results recorded. Results: A comparison of primary root length and lateral root number revealed that the greatest alterations in lateral root densities were observed for peptide sequences of the type GGGPSGS, whether or not serine was protected by t-butyl. Differences were also observed for these peptide sequences according to the identity of Y in the ~COCH2YCH2CO~ chain. Conclusion: The presence of serine’s oxygen atoms on the C-terminal side of the heptapeptide interact with Cl¯ leading to a change in ion concentrations and alterations in primary root lengths and lateral root densities.