Monomethylation of Lysine 20 on Histone H4 Facilitates Chromatin Maturation
Monomethylation of Lysine 20 on Histone H4 Facilitates Chromatin Maturation
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DOI：
10.1128/mcb.00989-08
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发表时间：
2008-11
影响因子：
5.3
通讯作者：
Annette N. D. Scharf;Karin Meier;Volker Seitz;E. Kremmer;A. Brehm;A. Imhof
中科院分区：
文献类型：
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作者：
Annette N. D. Scharf;Karin Meier;Volker Seitz;E. Kremmer;A. Brehm;A. Imhof
组蛋白修饰在形成染色质结构中起着重要作用。在这里，我们描述了使用果蝇胚胎提取物的体外染色质组装系统来研究组蛋白沉积后组蛋白修饰的动态变化。根据在体内观察到的情况，我们发现组蛋白H4最初的二乙酰化异构体发生了去乙酰化，这依赖于染色质组装。在组蛋白沉积到DNA上后，H4在K20处被单甲基化，这是H4分子有效去乙酰化所必需的。H4K20甲基化依赖性dl(3)MBT与染色质的关联以及dl(3)MBT- drpd3复合物的鉴定表明，脱乙酰酶通过与dl(3)MBT的相互作用特异性募集到单甲基化底物上。我们的数据表明，组蛋白修饰在染色质组装过程中以高度调控的方式被添加和移除。
ABSTRACT Histone modifications play an important role in shaping chromatin structure. Here, we describe the use of an in vitro chromatin assembly system from Drosophila embryo extracts to investigate the dynamic changes of histone modifications subsequent to histone deposition. In accordance with what has been observed in vivo, we find a deacetylation of the initially diacetylated isoform of histone H4, which is dependent on chromatin assembly. Immediately after deposition of the histones onto DNA, H4 is monomethylated at K20, which is required for an efficient deacetylation of the H4 molecule. H4K20 methylation-dependent dl(3)MBT association with chromatin and the identification of a dl(3)MBT-dRPD3 complex suggest that a deacetylase is specifically recruited to the monomethylated substrate through interaction with dl(3)MBT. Our data demonstrate that histone modifications are added and removed during chromatin assembly in a highly regulated manner.