Development of a regenerable cell culture system that senses and releases dead cells
Development of a regenerable cell culture system that senses and releases dead cells
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DOI：
10.1021/la046994e
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发表时间：
2005-04-26
期刊：
影响因子：
3.9
通讯作者：
Yamaguchi, T
中科院分区：
文献类型：
--
作者：
Okajima, S;Sakai, Y;Yamaguchi, T
我们开发了一种可快速再生的细胞培养系统，其中细胞培养基质检测细胞死亡并选择性地释放死细胞。这种培养材料是通过组合响应死细胞信号的检测器和释放死细胞的致动器来实现的。以苯并-18-冠-6-丙烯酰胺（BCAm）为受体，N-异丙基丙烯酰胺（NIPAM）为驱动器，研究了BCAm与冠醚受体的相互作用。这种NIPAM和BCAm的共聚物可以响应钾离子，并在37 ℃的培养温度下将其性质从疏水性变为亲水性。活细胞在内部浓缩钾离子;当细胞死亡时，钾离子被释放出来。聚合物表面识别从死细胞释放的钾离子，NIPAM水合，并且死细胞被选择性地分离。这种体外培养系统是一种新的系统，其中人工培养材料通过响应来自细胞的这种信号与细胞代谢协同工作，从而实现部分模拟体内稳态机制的体外组织再生。
We developed a rapidly regenerable cell culture system in which the cell culture substrate detects cell death and selectively releases the dead cells. This culture material was achieved by combining a detector that responds to the signal from the dead cells and an actuator to release the dead cells. Benzo-18-crown-6-acrylamide (BCAm) with a pendant crown ether receptor was used as the sensor to recognize cellular signals and N-isopropylacrylamide (NIPAM) was used as the actuator. This copolymer of NIPAM and BCAm can respond to potassium ions and change its nature from hydrophobic to hydrophilic at the culture temperature of 37 degrees C. Living cells concentrate potassium ion internally; when cells die, potassium ions are released. The polymer surface recognizes the potassium ions released from the dead cells, the NIPAM hydrates, and the dead cells are selectively detached. This in vitro culture system is a novel one in which artificial culture materials work cooperatively with cellular metabolism by responding to this signal from the cells, thereby realizing in vitro tissue regeneration partly mimicking the mechanisms of in vivo homeostasis.