Cycle-related toxicity and transformation in 10T1/2 cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.
Cycle-related toxicity and transformation in 10T1/2 cells treated with N-methyl-N'-nitro-N-nitrosoguanidine.
复制标题
用 N-甲基-N-硝基-N-亚硝基胍处理的 10T1/2 细胞中的循环相关毒性和转化。
DOI：
10.1073/pnas.77.8.4813
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发表时间：
1980
影响因子：
11.1
通讯作者：
Smith,GJ
中科院分区：
文献类型：
--
作者：
Grisham,JW;Greenberg,DS;Kaufman,DG;Smith,GJ
C_3H_（10）T_（1/2）Cl_8细胞在细胞周期的不同时间点暴露于不同浓度的N-甲基-N ′-硝基-N-亚硝基胍（MNNG）30分钟，引起剂量依赖性的毒性作用（相对集落形成效率或“存活”的降低），其在第一G1期期间线性增加，S期早中期达到最大值，S期晚下降。在第二个S期的过程中，毒性再次变得最大。转化率（III型病灶）以类似的模式增加和减少，在第一个G1期增加到S期早期的最大值，随后减少，然后在第二个S期再次增加。虽然最大毒性和转化的时期大致与S期的某些部分一致，但这些现象背后的机制似乎因以下原因而不同：（a）毒性与MNNG剂量线性相关，而后者与转化率的对数线性相关，以及（B）毒性和转化之间的比率随周期阶段和MNNG剂量而变化。
Exposure of C3H 10T1/2 Cl 8 cells, synchronized by release from confluence-induced arest of proliferation, to different concentrations of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for 30 min at various points during the cell cycle causes dose-dependent toxicity (decrease in relative colony-forming efficiency or "survival") that increases linearly during the first G1 phase, reaches a maximum in early to middle S phase, and decreases during late S. In the course of the second S phase, toxicity again becomes maximal. The transformation rate (type III foci) increases and decreases with a similar pattern, increasing during the first G1 phase to a maximum during early S phase, subsequently decreasing, and then increasing again during the second S phase. Although periods of maximal toxicity and transformation roughly coincide with some portion of the S phase, the mechanisms underlying these phenomena appear to differ for the following reasons: (a) toxicity is linearly related to dose of MNNG, whereas the latter is linearly related to the logarithm of transformation rate, and (b) the ratio between toxicity and transformation varies with the cycle phase and the dose of MNNG.