Getting into chromatin: how do transcription factors get past the histones?
Getting into chromatin: how do transcription factors get past the histones?
复制标题
DOI：
10.1139/o03-039
复制
发表时间：
2003-06-01
影响因子：
2.9
通讯作者：
Morse, RH
中科院分区：
文献类型：
--
作者：
Morse, RH
转录激活剂和一般转录机制必须能够接触到真核生物中可能被包装成核小体的DNA。在这篇综述中，我从这些DNA结合蛋白可能遇到的染色质结构的类型以及它们可能与各种染色质结构竞争的机制的角度来讨论这一问题。讨论包括在体内操纵染色质结构以对抗激活剂与核小体结合部位的实验，以及核小体动力学和激活结构域在促进进入这些部位中的作用。最后，我们讨论了激活剂在促进一般转录机制进入染色质部位的作用。
Transcriptional activators and the general transcription machinery must gain access to DNA that in eukaryotes may be packaged into nucleosomes. In this review, I discuss this problem from the standpoint of the types of chromatin structures that these DNA-binding proteins may encounter, and the mechanisms by which they may contend with various chromatin structures. The discussion includes consideration of experiments in which chromatin structure is manipulated in vivo to confront activators with nucleosomal binding sites, and the roles of nucleosome dynamics and activation domains in facilitating access to such sites. Finally, the role of activators in facilitating access of the general transcriptional machinery to sites in chromatin is discussed.