Functional profile of CD4+ and CD8+ T cells in latently infected individuals and patients with active TB
Functional profile of CD4+ and CD8+ T cells in latently infected individuals and patients with active TB
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DOI：
10.1016/j.tube.2012.12.002
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发表时间：
2013-03-01
期刊：
影响因子：
3.2
通讯作者：
Garcia, Luis F.
中科院分区：
文献类型：
--
作者：
Marin, Nancy D.;Paris, Sara C.;Garcia, Luis F.
结核病是世界范围内最重要的传染病之一。几项研究的重点是确定预防结核病的相关因素。他们中的大多数集中在干扰素-γ的研究上，因为它与预防结核病有很强的联系。然而，考虑到结核分枝杆菌感染后引起的免疫反应的复杂性，也应该考虑其他细胞因子。在本研究中，我们评估了Th1和Th17的反应及其与活动性疾病预防或发展的关系。因此，对非感染者(Non Tbi)、潜伏感染者(LTbi)和活动性肺结核患者(ATB)进行了研究。ELISPOT对细胞因子产生细胞数量的评估显示，ATB患者产生干扰素-γ的细胞数量较多，但在产生IL-17的细胞数量方面，研究组之间没有发现差异。在分枝杆菌抗原刺激的第1天和第6天，对产生CD4+和CD8+T细胞的干扰素-γ、IL-2、肿瘤坏死因子-α和IL-17的评估表明，存在与潜伏期或活动性结核病相关的功能特征。这里提出的结果表明，可能使用Th1型细胞因子的评估，如干扰素-γ和/或肿瘤坏死因子-α，作为预防结核病的相关性；然而，这些结果需要在其他组进行验证。(C)2013爱思唯尔有限公司。保留所有权利。
Tuberculosis (TB) is one of the most important infectious diseases around the world. Several studies have focused on the identification of correlates of protection against TB. Most of them have concentrated on the study of IFN-gamma due to its robust association with protection against TB. However, given the complexity of the immune response elicited after Mtb infection, other cytokines should also be considered. In the present study, we evaluated Th1 and Th17 responses and their association with the protection or development of active disease. Therefore, non infected individuals (nonTBi), latently infected individuals (LTBi) and patients with active TB (ATB) were studied. The evaluation of the number of cytokine producing cells by ELISPOT showed a higher number of IFN-gamma-producing cells in ATB patients, but no differences were found regarding the number of IL-17 producing cells among studied groups. The evaluation of IFN-gamma, IL-2, TNF-alpha and IL-17 producing CD4+ and CD8+ T cells at 1 day and 6 days of stimulation with mycobacterial antigens suggests the presence of functional signatures associated with latency or active TB. The results presented herein suggest the possible use of the evaluation of Th1-type cytokines, such as IFN-gamma and/or TNF-alpha, as a correlate of protection against TB; however, these results need to be validated for other groups. (C) 2013 Elsevier Ltd. All rights reserved.