Structure of the Yeast Mitochondrial Large Ribosomal Subunit
Structure of the Yeast Mitochondrial Large Ribosomal Subunit
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DOI：
10.1126/science.1249410
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发表时间：
2014-03-28
期刊：
影响因子：
56.9
通讯作者：
Ramakrishnan, V.
中科院分区：
文献类型：
--
作者：
Amunts, Alexey;Brown, Alan;Ramakrishnan, V.
线粒体有专门的核糖体，从它们的细菌和细胞质对应物中分离出来。我们已经解决了酵母线粒体大亚基的结构，使用单粒子冷冻电子显微镜。3.2埃的分辨率使一个几乎完整的原子模型能够从头构建和完善，包括39种蛋白质，其中13种是线粒体所特有的，以及线粒体RNA的扩展片段。该结构揭示了一个新的出口隧道路径和架构，E网站的独特元素，以及一个假定的膜对接网站。
Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.