Mitosis and protein synthesis 4 turnover of short- and long-lived proteins during the cell cycle of synchronized L-132 and HeLa S-3 cells.
Mitosis and protein synthesis 4 turnover of short- and long-lived proteins during the cell cycle of synchronized L-132 and HeLa S-3 cells.
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有丝分裂和蛋白质合成 4 同步 L-132 和 HeLa S-3 细胞的细胞周期期间短寿命和长寿命蛋白质的周转。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
S. Smith
中科院分区：
文献类型：
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作者：
D. Wheatley;E. Knecht;J. Cervera;S. Smith
在两个已建立的哺乳动物细胞系L-132和HeLa S-3中，采用选择和诱导同步的方法测量了在整个细胞周期中短寿命和长寿命蛋白质的周转率。在细胞周期的所有阶段，短暂的、新合成的蛋白质以相同的速率翻转，这一速率表示为Chase开始时存在的蛋白质总标记的百分比。由于M期蛋白质较少，其绝对周转率在分裂过程中可能有较小幅度的降低，说明合成与降解密切相关。相比之下，长寿蛋白的周转率明显降低，特别是在M期。长寿命蛋白质降解减少的一个可能的解释是在分裂过程中溶酶体活性受到抑制，而对由于非溶酶体系统而导致的短寿命蛋白质的周转没有明显的影响。
Turnover rates of short- and long-lived proteins throughout the cell cycle were measured in two established mammalian cell lines, L-132 and HeLa S-3, using both selection and induction synchronization. Short-lived, newly synthesized proteins turned over at the same rate during all stages of the cell cycle when this rate was expressed as a percentage of total labelling of proteins present at the start of the chase. Since fewer proteins were made in M-phase, the absolute turnover rate was probably reduced to a small degree during division itself, indicating a close co-ordination between synthesis and degradation. In contrast, long-lived proteins showed a considerable reduction in their rate of turnover specifically during M-phase. One possible explanation for the reduced degradation of long-lived protein is the suppression of lysosomal activity during division, with no apparent effect on the turnover of short-lived proteins which is due to a non-lysosomal system.