A simple non-invasive procedure for the investigation of cytochrome P-450 IIIA dependent enzymes in humans.
A simple non-invasive procedure for the investigation of cytochrome P-450 IIIA dependent enzymes in humans.
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发表时间：
1991-11
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通讯作者：
T. Bienvenu;E. Rey;G. Pons;P. D'athis;G. Olive
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作者：
T. Bienvenu;E. Rey;G. Pons;P. D'athis;G. Olive
尿6 β-羟基皮质醇（6 β OHF）是诱导细胞色素P-450 IIIA的特异性标志物。通常，6 β OHF的产生由24小时尿液收集表示为6 β OHF/17-羟基皮质类固醇的比率，或者甚至更好地表示为6 β OHF/游离皮质醇的比率，以调整肾上腺皮质醇产生的微小的每日变化。24小时尿液采集是一种适用于成人临床研究的无创方法，但在新生儿和婴儿中更难实现。因此，我们测试了单次尿样测定6 β OHF/游离皮质醇比值作为估计细胞色素P-450 IIIA酶活性的临床试验的有效性。用同样的提取方法，用高效液相色谱法测定了19名健康志愿者的尿6 β-OHF和游离皮质醇。晨尿与24小时尿6 β-OHF/游离皮质醇比值有较好的相关性（r = 0.899）。因此，晨点尿采样是一个简单的程序，可能有助于调查细胞色素P-450 IIIA酶的活性在人类，特别是在新生儿和儿童。
Urinary 6 beta-hydroxycortisol (6 beta OHF) is a specific marker of the induction of the cytochrome P-450 IIIA. Often, the production of 6 beta OHF is expressed from 24-h urine collection as the ratio 6 beta OHF/17-hydroxycorticosteroids or even better 6 beta OHF/free cortisol in order to adjust for minor day to day variation in adrenal cortisol production. A 24-h urine collection is a non-invasive method applicable to clinical studies in adult man, but more difficult to realize in newborn and infants. Therefore, we have tested the validity of a single urine sampling for the determination of the 6 beta OHF/free cortisol ratio as a clinical test to estimate cytochrome P-450 IIIA enzyme activity. Urinary 6 beta OHF and free cortisol have been measured in 19 healthy volunteers by high performance liquid chromatography methods using the same extraction procedure. A good correlation was found between 6 beta OHF/free cortisol ratio in morning urine samples and in 24h-collections (r = 0.899). Thus, a morning spot urine sampling is a simple procedure that may be helpful for investigations on cytochrome P-450 IIIA enzyme activity in humans, particularly in newborn and in children.