Cytokine polymorphisms in men with chronic prostatitis/chronic pelvic pain syndrome: Association with diagnosis and treatment response
Cytokine polymorphisms in men with chronic prostatitis/chronic pelvic pain syndrome: Association with diagnosis and treatment response
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DOI：
10.1016/s0022-5347(05)64916-6
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发表时间：
2002-07-01
影响因子：
6.6
通讯作者：
Cook, D
中科院分区：
文献类型：
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作者：
Shoskes, DA;Albakri, Q;Cook, D
目的：慢性盆腔疼痛综合征是一种常见的病因不明的疾病。前列腺液和精液中的细胞因子升高是常见的发现。我们研究了遗传多态性，可以改变细胞因子基因表达的男性与慢性盆腔疼痛syndrome.Materials和方法：基因组DNA从血液中提取36名男性慢性盆腔疼痛syndrome。使用反向序列特异性寡核苷酸探测对细胞因子启动子位点的多态性进行基因分型，即肿瘤坏死因子（TNF）-α 308、转化生长因子（TGF）-β 25、TGF-β 10、白细胞介素（IL）-10 1082和IL-6 174。基因型频率与252名对照组以及根据诊断类别和治疗反应的慢性盆腔疼痛综合征患者组进行比较。患有慢性盆腔疼痛综合征的男性和对照患者的TNF-α、TGF-β或IL-6等位基因频率没有差异，尽管慢性盆腔疼痛综合征患者更可能表达与低IL-10产生相关的基因型（30.6%对12.1%，p = 0.007）。当比较美国国立卫生研究院的诊断时，IIIa类患者更可能具有低TNF-α基因型（II类，IIIa类和IIIb类分别为33%，100%和18%，p = 0.04）。28例接受抗炎槲皮素治疗的患者中，治疗失败的11例患者的TNF-α基因型较低，而治疗成功的患者中有29.4%的患者TNF-α基因型较低（p = 0.0003）。同样，槲皮素治疗失败的男性比治疗成功的男性更不可能有低IL-10基因型（9.1%对47.1%，p = 0.04）。结论：慢性盆腔疼痛综合征患者更可能有低IL-10产生基因型，这表明自身免疫是一种潜在的病因。抗炎植物治疗失败与低TNF-α和高IL-10表型相关，这可能有助于定义一个没有炎症病因的慢性盆腔疼痛综合征患者亚组。
Purpose: The chronic pelvic pain syndrome is a common disorder of unknown etiology. Elevated cytokines in prostate fluid and semen are frequent findings. We studied genetic polymorphisms that can alter cytokine gene expression in men with the chronic pelvic pain syndrome.Materials and Methods: Genomic DNA was extracted from blood from 36 men with the chronic pelvic pain syndrome. Reversed sequence specific oligonucleotide probing was used to genotype the polymorphisms for cytokine promoter sites, namely tumor necrosis factor (TNF)-alpha 308, transforming growth factor (TGF)-beta 25, TGF-beta 10, interleukin (IL)-10 1082 and IL-6 174. Genotype frequencies were compared with 252 controls as well as among groups of patients with the chronic pelvic pain syndrome according to diagnostic category and treatment response.Results: There were no differences in men with the chronic pelvic pain syndrome and control patients in the frequency of TNF-alpha, TGF-beta or IL-6 alleles, although those with the chronic pelvic pain syndrome were more likely to express the genotype associated with low IL-10 production (30.6% versus 12.1%, p = 0.007). When comparing National Institutes of Health diagnoses, category IIIa patients were more likely to have the low TNF-alpha genotype (categories II, IIIa and IIIb 33%, 100% and 18%, respectively, p = 0.04). All 11 of the 28 patients treated with the anti-inflammatory quercetin in whom treatment failed had the low TNF-alpha genotype versus 29.4% of those in whom treatment succeeded (p = 0.0003). Similarly men with quercetin treatment failure were much less likely to have the low IL-10 genotype than those with treatment success (9.1% versus 47.1%, p = 0.04).Conclusions: Patients with the chronic pelvic pain syndrome are more likely to have a low IL-10 producing genotype, suggesting autoimmunity as a potential etiology. Anti-inflammatory phytotherapy failure was associated with low TNF-alpha and high IL-10 phenotypes, which may help define a subset of patients with the chronic pelvic pain syndrome without an inflammatory etiology.