LACK OF CORRELATION BETWEEN THE ACCUMULATION OF PLUS-STRAND LEADER RNA AND THE INHIBITION OF PROTEIN AND RNA-SYNTHESIS IN VESICULAR STOMATITIS-VIRUS INFECTED-MOUSE L-CELLS
LACK OF CORRELATION BETWEEN THE ACCUMULATION OF PLUS-STRAND LEADER RNA AND THE INHIBITION OF PROTEIN AND RNA-SYNTHESIS IN VESICULAR STOMATITIS-VIRUS INFECTED-MOUSE L-CELLS
复制标题
DOI：
10.1016/0042-6822(86)90282-5
复制
发表时间：
1986-04-15
期刊：
影响因子：
3.7
通讯作者：
LUCASLENARD, J
中科院分区：
文献类型：
--
作者：
DUNIGAN, DD;BAIRD, S;LUCASLENARD, J
抑制水泡性口炎病毒（VSV）感染的小鼠L细胞中的蛋白质合成需要表达的病毒基因组的两个区域（一个大的和一个小的），通过目标大小分析确定。宿主RNA合成的抑制也显示依赖于VSV基因组的两个区域的表达，最有可能是相同的。在某些情况下，例如在被突变体T1026R1或tsG41感染的细胞中，或中等强度紫外线照射的VSV，仅表达其中一个区域，但细胞蛋白质和RNA合成减少。这表明病毒基因组每个区域的产物可以独立地起作用。在这些情况下，抑制的严重程度取决于感染期的长度和感染的多重性。两种基因产物的身份都不清楚，但有人认为小产物是正链前导RNA。然而，如本文所示，宿主大分子合成抑制的程度与感染细胞中前导RNA的量之间没有相关性。
The inhibition of protein synthesis in mouse L cells infected by vesicular stomatitis virus (VSV) requires expression of two regions (one large and one small) of the viral genome, as determined by target size analysis. The inhibition of host RNA synthesis was also shown to be dependent on expression of two regions of the VSV genome, most likely the same ones. In some cases, such as in cells infected by mutants T1026R1, or tsG41 at 40.degree., or moderately uv irridiated VSV, only one of the two regions was expressed, yet cellular protein and RNA synthesis was decreased. This suggests that the product of each region of the viral genome can act independently. In these instances the severity of the inhibition was dependent on both the length of the infection period and the multiplicity of infection The identity of neither gene product is known, but it has been suggested that small product is plus-strand leader RNA. As shown herein, however, there was no correlation between the extent of host macromolecular synthesis inhibition and the quantity of leader RNA in infected cells.