Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei.
Timing of nuclear and kinetoplast DNA replication and early morphological events in the cell cycle of Trypanosoma brucei.
复制标题
布氏锥虫细胞周期中核和动质体 DNA 复制的时间和早期形态事件。
作者：
R. Woodward;K. Gull
我们已经使用免疫荧光检测5-溴-2-脱氧尿苷取代的DNA，以确定启动的时间和持续时间的核和动基体S期内的布氏锥虫细胞周期的procyclic阶段。细胞核和动基体S期均呈周期性，分别占细胞周期的0.18和0.12。此外，这两个S期的启动是在近似同步，相差只有0.03的单位细胞周期。我们还使用了一种单克隆抗体，它可以识别T. brucei，以便使具有新的原基底体的细胞可视化，从而确定细胞周期内原基底体形成的时间。亲基体形成发生在几分钟内的核S期开始，在0.41的单位细胞周期。这提供了T细胞中已知最早的细胞周期事件的检测。在光学显微镜下观察布鲁氏菌。细胞周期事件，包括核和动基体DNA复制的启动和前基体的形成可能是严格协调的T。brucei，以维持间期细胞精确的单鞭毛（动基体）、单鞭毛状态。
We have used immunofluorescent detection of 5-bromo-2-deoxyuridine-substituted DNA in order to determine the timing of initiation and the duration of nuclear and kinetoplast S-phases within the procyclic stage of the Trypanosoma brucei cell cycle. Both nuclear and kinetoplast S-phases were shown to be periodic, occupying 0.18 and 0.12 of the unit cell cycle, respectively. In addition, initiation of both of these S-phases were in approximate synchrony, differing by only 0.03 of the unit cell cycle. We have also used a monoclonal antibody that recognises the basal bodies of T. brucei in order to visualise cells possessing a new pro-basal body and hence determine the time of pro-basal body formation within the cell cycle. Pro-basal body formation occurred within a few minutes of the initiation of nuclear S-phase, at 0.41 of the unit cell cycle. This provides detection of the earliest known cell cycle event in T. brucei at the level of the light microscope. Cell cycle events including initiation of nuclear and kinetoplast DNA replication and pro-basal body formation may be strictly coordinated in T. brucei in order to maintain the precise single-mitochondrion (kinetoplast), singleflagellum status of the interphase cell.