LOW-DENSITY-LIPOPROTEIN RICH IN OLEIC-ACID IS PROTECTED AGAINST OXIDATIVE MODIFICATION - IMPLICATIONS FOR DIETARY PREVENTION OF ATHEROSCLEROSIS
LOW-DENSITY-LIPOPROTEIN RICH IN OLEIC-ACID IS PROTECTED AGAINST OXIDATIVE MODIFICATION - IMPLICATIONS FOR DIETARY PREVENTION OF ATHEROSCLEROSIS
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DOI：
10.1073/pnas.87.10.3894
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发表时间：
1990-05-01
影响因子：
11.1
通讯作者：
STEINBERG, D
中科院分区：
文献类型：
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作者：
PARTHASARATHY, S;KHOO, JC;STEINBERG, D
低密度脂蛋白（LDL）的氧化修饰以多种方式增强其潜在的致动脉粥样硬化性，特别是通过增强巨噬细胞的摄取。兔子体内研究表明，抑制 LDL 氧化可减缓动脉粥样硬化病变的进展。在本研究中，给兔子喂食的是新开发的变体葵花籽油（Trisun 80）（含有超过 80% 的油酸和仅 8% 的亚油酸），或传统的葵花籽油（仅含有 20% 的油酸和 67% 的亚油酸）。从喂食变种葵花籽油的动物血浆中分离出的 LDL 油酸含量很高，亚油酸含量非常低。这些富含油酸的低密度脂蛋白颗粒对氧化修饰具有显着的抵抗力。即使暴露于铜诱导的氧化 16 小时或与培养的内皮细胞孵育 24 小时后，巨噬细胞对 LDL 的摄取也仅略有增强。结果表明，富含油酸的饮食除了具有降低 LDL 的作用外，还可以通过产生对氧化修饰具有高度抵抗力的 LDL 来减缓动脉粥样硬化的进展。
Oxidative modification of low density lipoprotein (LDL) enhances its potential atherogenicity in several ways, notably by enhancing its uptake into macrophages. In vivo studies in the rabbit show that inhibition of LDL oxidation slows the progression of atherosclerotic lesions. In the present studies, rabbits were fed either a newly developed variant sunflower oil (Trisun 80), containing more than 80% oleic acid and only 8% linoleic acid, or conventional sunflower oil, containing only 20% oleic acid and 67% linoleic acid. LDL isolated from the plasma of animals fed the variant sunflower oil was highly enriched in oleic acid and very low in linoleic acid. These oleate-rich LDL particles were remarkably resistant to oxidative modification. Even after 16-hr exposure to copper-induced oxidation or 24-hr incubation with cultured endothelial cells, macrophage uptake of the LDL was only marginally enhanced. The results suggest that diets sufficiently enriched in oleic acid, in addition to their LDL-lowering effect, may slow the progression of atherosclerosis by generating LDL that is highly resistant to oxidative modification.