Multiple Forms of Glucoamylase of Rhizopus Species
Multiple Forms of Glucoamylase of Rhizopus Species
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根霉属物种的多种形式的葡糖淀粉酶
DOI：
10.1002/star.19750270407
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
S. Kano
中科院分区：
文献类型：
--
作者：
S. Ueda;S. Kano
采用玉米淀粉吸附技术和CM-纤维素层析技术，将根霉的糖化酶系统分离为两种糖化酶。这些级分之一，称为葡糖淀粉酶I，具有很强的脱支活性，并且在生淀粉消化中具有高活性。
添加根霉和曲霉的α-淀粉酶可加速糖化酶Ⅰ对糯玉米原淀粉的消化。糖化酶I对非糯玉米原淀粉的消化比糯玉米原淀粉困难，添加曲霉α-淀粉酶后，非糯玉米原淀粉的消化速度加快。
另一方面，另一个组分，称为葡糖淀粉酶II，具有弱的脱支活性，不能吸附在生淀粉上。糖化酶II对糯玉米原淀粉的消化作用很弱，而添加根霉α-淀粉酶则能促进淀粉的消化。
The glucoamylase system of Rhizopus sp. was fractionated into two kinds of glucoamylase by using the corn starch adsorption technique supplemented by CM-cellulose chromatography. One of these fractions, referred to as glucoamylase I, has a strong debranching activity and is highly active in raw starch digestion.
The raw waxy corn starch digestion by glucoamylase I was accelerated by adding a-amylase from Rhizopus sp. or from Aspergillus oryzae. The raw non-waxy corn starch digestion by glucoamylase I was more difficult than the raw waxy corn starch digestion, which was also accelerated by adding α-amylase from Aspergillus oryzae.
On the other hand, another fraction, referred to as glucoamylase II, has a weak debranching activity and can not be adsorbed on raw starch. The raw waxy corn starch digestion by glucoamylase II was very weak, but was accelerated by adding α-amylase from Rhizopus sp.