CHANGES IN CARDIAC AND HEPATIC GLUCOCORTICOID RECEPTORS AFTER ADRENALECTOMY
CHANGES IN CARDIAC AND HEPATIC GLUCOCORTICOID RECEPTORS AFTER ADRENALECTOMY
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DOI：
10.1042/cs0510487
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
MEYER, P
中科院分区：
文献类型：
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作者：
GREGORY, MC;DUVAL, D;MEYER, P
肾上腺切除后，每隔一段时间测量大鼠心脏和肝脏胞浆中地塞米松特异性结合部位的总数。肾上腺切除后12~48h，心、肝胞浆液中结合位点数明显增加。亲和力没有变化。静脉注射后与肝胞浆蛋白结合的[~3H]皮质酮。体内注射后2小时内消失率达98%。因此，在受体数量增加前几个小时，内源性皮质酮受体复合体在肝胞浆中的解离将基本完成。糖皮质激素受体的数量确实增加，主要发生在内源性类固醇抑制后12至48小时之间。
The total number of specific dexamethasone-binding sites in rat heart and liver cytosol was measured at intervals after adrenalectomy. Between 12 and 48 h after adrenalectomy there was a significant increase in the number of binding sites in both heart and liver cytosol. Affinity was unchanged. Of the [3H]corticosterone bound to liver cytosol proteins after an i.v. injection 98% disappeared within 2 h in vivo. Dissociation of endogenous corticosterone-receptor complexes in liver cytosol will thus be substantially complete some hours before the number of receptors increases. There is a true increase in the number of glucocorticoid receptors occurring principally between 12 and 48 h after suppression of endogenous steroids.