Localized electroporation and molecular delivery into single living cells by atomic force microscopy
Localized electroporation and molecular delivery into single living cells by atomic force microscopy
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DOI：
10.1063/1.2981568
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发表时间：
2008-10-13
影响因子：
4
通讯作者：
Wickramasinghe, H. Kumar
中科院分区：
文献类型：
--
作者：
Nawarathna, D.;Unal, K.;Wickramasinghe, H. Kumar
我们提出了一种高效、快速的方法，使用改进的原子力显微镜尖端对数百万至数十个细胞中的单个活细胞进行选择性和局部电穿孔。使用倒置显微镜实时观察电穿孔。该技术被提议作为生物分子、蛋白质、药物和基因的有效和受控递送的工具。 (C) 2008 年美国物理研究所。 [DOI：10.1063/1.2981568]
We present an efficient and fast method for selective and localized electroporation of a single living cell from a population of millions to tens of cells using the modified tip of an atomic force microscope. Electroporation was observed in real time using an inverted microscope. This technique is proposed as a tool for efficient and controlled delivery of biomolecules, proteins, drugs, and genes. (C) 2008 American Institute of Physics. [DOI: 10.1063/1.2981568]