miR-219-5p targets TBXT and inhibits breast cancer cell EMT and cell migration and invasion.
miR-219-5p targets TBXT and inhibits breast cancer cell EMT and cell migration and invasion.
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miR-219-5p靶向TBXT并抑制乳腺癌细胞EMT和细胞迁移和侵袭
DOI：
10.1042/bsr20210318
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发表时间：
2021-08-27
影响因子：
4
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Ye Q;Wang X;Yuan M;Cui S;Chen Y;Hu Z;Liu D;Han C;Li B;Chen D
据报道，miR-219- 5 p通过靶向不同的基因在不同的癌症中充当肿瘤抑制剂或肿瘤促进剂。在本研究中，我们证明了miR-219- 5 p通过直接结合TBXT 3′-非翻译区来负调控TBXT的表达，TBXT是一种已知的上皮-间质转化（EMT）诱导剂。由于其对TBXT表达的抑制，miR-219- 5 p抑制乳腺癌细胞中的EMT和细胞迁移和侵袭。在miR-219- 5 p过表达的细胞中重新引入TBXT降低了miR-219对EMT和细胞迁移和侵袭的抑制作用。此外，miR-219- 5 p降低了乳腺癌干细胞（CSC）标记基因的表达，并降低了细胞的乳腺球形成能力。总体而言，我们的研究强调TBXT是miR-219- 5 p的新靶点。通过抑制TBXT，miR-219- 5 p在乳腺癌细胞的EMT和细胞迁移和侵袭中起重要作用。
miR-219-5p has been reported to act as either a tumor suppressor or a tumor promoter in different cancers by targeting different genes. In the present study, we demonstrated that miR-219-5p negatively regulated the expression of TBXT, a known epithelial–mesenchymal transition (EMT) inducer, by directly binding to TBXT 3′-untranslated region. As a result of its inhibition on TBXT expression, miR-219-5p suppressed EMT and cell migration and invasion in breast cancer cells. The re-introduction of TBXT in miR-219-5p overexpressing cells decreased the inhibitory effects of miR-219 on EMT and cell migration and invasion. Moreover, miR-219-5p decreased breast cancer stem cell (CSC) marker genes expression and reduced the mammosphere forming capability of cells. Overall, our study highlighted that TBXT is a novel target of miR-219-5p. By suppressing TBXT, miR-219-5p plays an important role in EMT and cell migration and invasion of breast cancer cells.